Immobilization of Enzymes and Cells

1. Front Matter

   Pages i-xiv

   PDF

2. Immobilization of Enzymes as the 21st Century Begins

   • Jose M. Guisan

   Pages 1-13

3. Immobilization of Enzymes

   • Beatriz M. Brena, Francisco Batista-Viera

   Pages 15-30

4. Cross-Linked Enzyme Aggregates

   • Roger A. Sheldon, Rob Schoevaart, Luuk M. van Langen

   Pages 31-45

5. Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

   • Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan et al.

   Pages 47-55

6. Glutaraldehyde in Protein Immobilization

   • Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente et al.

   Pages 57-64

7. Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques

   • Manfred T. Reetz

   Pages 65-76

8. Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

   • Mónica Campás, Jean-Louis Marty

   Pages 77-85

9. Design of Smart Biocatalysts

   • Ipsita Roy, Munishwar N. Gupta

   Pages 87-95

10. Affinity Immobilization of Tagged Enzymes

    • Silvana Andreescu, Bogdan Bucur, Jean-Louis Marty

    Pages 97-106

11. Bioaffinity Immobilization

    • Ipsita Roy, Munishwar N. Gupta

    Pages 107-116

12. One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

    • Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan et al.

    Pages 117-128

13. Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran

    • Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo et al.

    Pages 129-141

14. Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports

    • Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente et al.

    Pages 143-152

15. Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

    • Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes et al.

    Pages 153-162

16. Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports

    • Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor et al.

    Pages 163-173

17. Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach

    • Alankar Vaidya, Lutz Fischer

    Pages 175-183

18. Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups

    • Francisco Batista-Viera, Karen Ovsejevi, Carmen Manta

    Pages 185-204

19. Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers

    • Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego et al.

    Pages 205-216

20. Immobilization of Enzymes on Magnetic Particles

    • Martina Koneracká, Peter Kopčanský, Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan et al.

    Pages 217-228

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

• Activation
• Glucose
• enzyme
• enzymes
• protein

"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of immobilization...an excellent reference work that will have long lasting value." -Quarterly Review of Biology

"...one of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

"...an ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst

• Institute of Catalysis, CSIC, Campus UAM-Cantoblanco, Madrid, Spain

  Jose M. Guisan

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