Transmembrane Signaling Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Overviews

   1. Front Matter

      Pages 1-1

      PDF

   2. Transmembrane Signaling by G Protein-Coupled Receptors

      • Louis M. Luttrell

      Pages 3-49

   3. Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases

      • Kanchana Natarajan, Bradford C. Berk

      Pages 51-77

   4. Protein-Protein Interactions in Signaling Cascades

      • Bruce J. Mayer

      Pages 79-99

3. Specific Topics

   1. Front Matter

      Pages 101-101

      PDF

   2. Membrane Receptors and Signaling

      1. Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells

         • Marcin Majka, Mariusz Z. Ratajczak

         Pages 103-114

      2. Functional Expression of CXCR4 in <i>Saccharomyces cerevisiae</i> in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4

         • Zi-xuan Wang, James R. Broach, Stephen C. Peiper

         Pages 115-127

      3. Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors

         • Jean-Marc Navenot, Zi-xuan Wang, Stephen C. Peiper

         Pages 129-139

      4. G Protein-Coupled Receptor Dimerization and Signaling

         • Mario Mellado, Antonio Serrano, Carlos Mart’inez-A, José Miguel Rodríguez-Frade

         Pages 141-158

      5. Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells

         • Venkatakrishna R. Jala, Bodduluri Haribabu

         Pages 159-166

   3. Lipid Raft and Caveolae in Transmembrane Signaling

      1. Isolation of Membrane Rafts and Signaling Complexes

         • Kathleen Boesze-Battaglia

         Pages 169-179

      2. Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae

         • Rennolds S. Ostrom, Paul A. Insel

         Pages 181-190

   4. Protein—Protein Interaction in Transmembrane Signaling

      1. Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions

         • Tarik Issad, Ralf Jockers

         Pages 195-209

      2. Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen

         • Kelly L. Jordan-Sciutto, Marshall B. Montgomery

         Pages 211-231

      3. Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays

         • Hyun Woo Lee, Jaewon Ko, Eunjoon Kim

         Pages 233-244

      4. Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins

         • Jaewon Ko, Eunjoon Kim

         Pages 245-253

   5. Rho Guanosine Triphosphatases and Reconstitution of Signaling Complexes

      1. Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases

         • Ritu Garg, Anne J. Ridley

         Pages 257-267

      2. Affinity-Based Assay of Rho Guanosine Triphosphatase Activation

         • Mary Stofega, Celine DerMardirossian, Gary M. Bokoch

         Pages 269-279

      3. Assay of Phospholipase D Activity in Cell-Free Systems

         • Shankar S. Iyer, David J. Kusner

         Pages 281-298

The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways.

• Activation
• Proteomics
• Regulation
• Vivo
• gene expression
• genes
• membrane proteins
• molecular biology
• neutrophils
• regulation mechanisms
• saccharomyces cerevisiae
• signal transduction

From Reviews of the First Edition...

"...well put together...covers a range of signaling protocols and would serve well as a reference for what methods are currently being used in the field." -Molecular Biotechnology

"...a good book for a non-specialist entering the field and specialists looking for well-described techniques." -Microbiology Today

• Department of Pathology, School of Dental Medicine, The University of Pennsylvania, Philadelphia

  Hydar Ali

• Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville

  Bodduluri Haribabu

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