Embryonic Stem Cell Protocols

1. Front Matter

   Pages i-xxi

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2. Isolation and Maintenance

   1. Neural Stem Sphere Method

      • Takashi Nakayama, Nobuo Inoue

      Pages 1-13

   2. Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro

      • Nathalie Billon, Christine Jolicoeur, Martin Raff

      Pages 15-32

   3. Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer

      • Nibedita Lenka

      Pages 33-54

   4. Optimized Neuronal Differentiation of Murine Embryonic Stem Cells

      • Matthew T. Lorincz

      Pages 55-69

   5. Generation of Inner Ear Cell Types From Embryonic Stem Cells

      • Marcelo N. Rivolta, Huawei Li, Stefan Heller

      Pages 71-92

   6. Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures

      • Tammy-Claire Troy, Kursad Turksen

      Pages 93-104

   7. Directing Epidermal Fate Selection by a Novel Co-Culture System

      • Tammy-Claire Troy, Kursad Turksen

      Pages 105-111

3. Signaling in Embryonic Stem Cell Differentiation

   1. In Vitro Generation of T Lymphocytes From Embryonic Stem Cells

      • Renée F. de Pooter, Thomas M. Schmitt, Juan Carlos Zúñiga-Pflücker

      Pages 113-121

   2. The Role of Hex in Hemangioblast and Hematopoietic Development

      • Rebecca J. Chan, Robert Hromas, Mervin C. Yoder

      Pages 123-133

   3. Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells

      • Jitsutaro Kawaguchi

      Pages 135-148

   4. Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures

      • Nicole L. Woll, Sarah K. Bronson

      Pages 149-159

   5. Generation of Chondrocytes From Embryonic Stem Cells

      • Jaspal Singh Khillan

      Pages 161-170

   6. Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro

      • Jan Kramer, Gunnar Hargus, Jürgen Rohwedel

      Pages 171-190

   7. Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions

      • Cornelia Gissel, Michael Xavier Doss, Rita Hippler-Altenburg, Jürgen Hescheler, Agapios Sachinidis

      Pages 191-219

   8. Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes

      • Lijuan L. Shang, Samuel C. Dudley Jr., Arnold E. Pfahnl

      Pages 221-231

   9. Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro

      • Ali Samadikuchaksaraei, Anne E. Bishop

      Pages 233-248

4. Genetic Manipulation of Embryonic Stem Cells

   1. Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro

      • Reigh-Yi Lin, Terry F. Davies

      Pages 249-261

   2. Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells

      • Takatsugu Yamada, Yoshiyuki Nakajima

      Pages 263-278

   3. Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells

      • Shigeko Torihashi

      Pages 279-285

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

• Vivo
• biology
• cell
• cell culture
• cell differentiation
• development
• neural stem cells
• protein
• stem cell
• stem cells
• tissue

From Reviews of the First Edition...

"...elegantly introduces tremendous methods and protocols in ES studies...one of the most useful books that I have ever read in this field..." -Cell Biology International

"...highly valuable for any scientist who wants to make a start in the exciting field but also for experienced ES cell researchers who want to widen their repertoire" -Diabetologia

"...a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research" -Molecular Biotechnology

"...a useful companion volume to other more specialized ES cell books..." -Nature Cell Biology

• Ottawa Health Research Institute, Ottawa, Canada

  Kursad Turksen

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