Overview
- Resources for the analysis of gene regulation data and SNPs
- Comparative genomic approach for functionally annotating human DNA
- Quantitative, real-time RT-PCR in cardiovascular research
- In silico analysis of SNPs and other high-throughput data
- Conditional targeting in the myocardium
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 366)
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Table of contents (20 protocols)
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Front Matter
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Cardiac Gene Expression
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Front Matter
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In Silico Assessment of Regulatory cis-Elements and Gene Regulation
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Front Matter
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Cardiac Single Nucleotide Polymorphisms
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Front Matter
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Keywords
About this book
Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function.
Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.
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Editors and Affiliations
Bibliographic Information
Book Title: Cardiac Gene Expression
Book Subtitle: Methods and Protocols
Editors: Jun Zhang, Gregg Rokosh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-030-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-352-7Published: 19 March 2007
Softcover ISBN: 978-1-61737-514-9Published: 09 October 2011
eBook ISBN: 978-1-59745-030-0Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 366
Number of Illustrations: 65 b/w illustrations, 1 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Cardiology, Bioinformatics