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Organelle Proteomics

  • Book
  • © 2008

Overview

Editors:
  1. Delphine Pflieger

    1. Laboratoire de Neurobiologie et Diversité Cellulaire CNRS UMR 7637, Ecole Supérieure de Physique et de Chimie Industrielles, France
      Laboratoire d’Analyse et de Modélisation pour la Biologie et I’Environnement CNRS UMR 8587, Université d’Evry Val d’Essonne, Evry, France

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  2. Jean Rossier

    1. Laboratoire de Neurobiologie et Diversité Cellulaire CNRS UMR 7637, Ecole Supérieure de Physique et de Chimie Industrielles, France

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  • Collects time tested reproducible laboratory protocols including tricks and hints written by experts in the field
  • Currently the only book that examines organelle proteomics in depth

Part of the book series: Methods in Molecular Biology (MIMB, volume 432)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry

      • Edein P. Romijn, John R. Yates III
      Pages 1-16

  3. Analysis of Organelles, Vesicles, and Protein Machineries

    1. Front Matter

      Pages 17-17
      Download chapter PDF

    2. Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments

      • Daniel Salvi, Norbert Rolland, Jacques Joyard, Myriam Ferro
      Pages 19-36

    3. Proteomic Analysis of the Stacked Golgi Complex

      • Mark S. Lowenthal, Kathryn E. Howell, Christine C. Wu
      Pages 37-49

    4. Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device

      • Hans Zischka, Norbert Kinkl, Ralf J. Braun, Marius Ueffing
      Pages 51-64

    5. Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria

      • Claire Lemaire, Geneviève Dujardin
      Pages 65-81

    6. Mitochondrial Proteomics

      • Thierry Rabilloud
      Pages 83-100

    7. Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane

      • Sandrine Da Cruz, Jean-Claude Martinou
      Pages 101-116

    8. Subcellular Fractionation and Proteomics of Nuclear Envelopes

      • Laurence Florens, Nadia Korfali, Eric C. Schirmer
      Pages 117-137

    9. Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction

      • Tsuneyoshi Horigome, Kazuhiro Furukawa, Kohei Ishii
      Pages 139-148

    10. Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis

      • Kiichi Fukui, Hideaki Takata, Susumu Uchiyama
      Pages 149-160

    11. Purification and Proteomic Analysis of Plant Plasma Membranes

      • Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom, Christer Larsson
      Pages 161-173

    12. Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues

      • Jacek R Wiśniewski
      Pages 175-183

    13. Proteomic Characterization of Membrane Protein Topology

      • Adele R. Blackler, Christine C. Wu
      Pages 185-197

    14. Free Flow Isoelectric Focusing

      • Markus Islinger, Gerhard Weber
      Pages 199-215

    15. Use of Gas-Phase Fractionation to Increase Protein Identifications

      • Jacob Kennedy, Eugene C. Yi
      Pages 217-228

    16. Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins

      • Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan
      Pages 229-241

    17. Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification

      • Sylvie Kieffer– Jaquinod, Agnès Chapel, Jérôme Garin, Agnøs Journet
      Pages 243-258
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Keywords

About this book

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

Editors and Affiliations

  • Laboratoire de Neurobiologie et Diversité Cellulaire CNRS UMR 7637, Ecole Supérieure de Physique et de Chimie Industrielles, France

    Delphine Pflieger, Jean Rossier

  • Laboratoire d’Analyse et de Modélisation pour la Biologie et I’Environnement CNRS UMR 8587, Université d’Evry Val d’Essonne, Evry, France

    Delphine Pflieger

Bibliographic Information

  • Book Title: Organelle Proteomics

  • Editors: Delphine Pflieger, Jean Rossier

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-028-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-779-2Published: 19 February 2008

  • Softcover ISBN: 978-1-61737-772-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-028-7Published: 27 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 421

  • Topics: Biochemistry, general, Proteomics, Cell Biology

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