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Glycomics

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Niclas G. Karlsson
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    You can also search for this editor in PubMed   Google Scholar

  2. Nicolle H. Packer
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    You can also search for this editor in PubMed   Google Scholar

  • Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research
  • Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins
  • Disperses the misconception that only experts can analyze the glycome
  • Inspires researchers to pursue glycan function through sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 534)

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Table of contents (26 protocols)

  1. Front Matter

    Pages ii-xiv
    Download chapter PDF

  2. Glycan Analysis

    1. Front Matter

      Pages 1-2
      Download chapter PDF

    2. Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry

      • Willy Morelle, Valegh Faid, Frédéric Chirat, Jean-Claude Michalski
      Pages 3-21

    3. Infrared Multiphoton Dissociation Mass Spectrometry for Structural Elucidation of Oligosaccharides

      • Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B. Lebrilla
      Pages 23-35

    4. Mass Spectrometry of N-Linked Glycans

      • Parastoo Azadi, Christian Heiss
      Pages 37-51

    5. Solid-Phase Permethylation for Glycomic Analysis

      • Yehia Mechref, Pilsoo Kang, Milos V. Novotny
      Pages 53-64

    6. Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization

      • Erika Lattová, Hélène Perreault
      Pages 65-77

    7. Two-Dimensional HPLC Separation with Reverse-Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools After Labeling with 2-Aminobenzamide

      • Manfred Wuhrer, Carolien A.M. A. M. Koeleman, André M. Deelder
      Pages 79-91

    8. Capillary Lectin-Affinity Electrophoresis for Glycan Analysis

      • Kazuaki Kakehi, Mitsuhiro Kinoshita
      Pages 93-105

    9. Analysis of Methylated O-Glycan Alditols by Reversed-Phase NanoLC Coupled CAD-ESI Mass Spectrometry

      • Franz-Georg Hanisch, Stefan Müller
      Pages 107-115

    10. High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling

      • Hasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, Gunnar C. Hansson
      Pages 117-131

    11. Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans

      • Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B. Lebrilla
      Pages 133-145

    12. The Structural Elucidation of Glycosaminoglycans

      • Vikas Prabhakar, Ishan Capila, Ram Sasisekharan
      Pages 147-156

    13. Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS Separations

      • Mark Skidmore, Abdel Atrih, Ed Yates, Jeremy E. Turnbull
      Pages 157-169

    14. Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis

      • Ruby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, Niclas G. Karlsson
      Pages 171-192

  3. Glycopeptides

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Enrichment Strategies for Glycopeptides

      • Shigeyasu Ito, Ko Hayama, Jun Hirabayashi
      Pages 194-203

    3. Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting

      • Nicole Wilson, Raina Simpson, Catherine Cooper-Liddell
      Pages 205-212

    4. Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF

      • Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, Tillman Gerngross
      Pages 213-223

    5. N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS

      • Urs Lewandrowski, Albert Sickmann
      Pages 225-238
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Keywords

About this book

Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.

Reviews

From the reviews: “This book discusses methods of a comparatively new direction in science, which has been named glycomics. … The book consists two parts with 26 chapters prepared by well-known experts from many countries. … I confirm the book fulfills the goal and I consider this book as an excellent practical guide to glycomics methods. Each method is described in great detail, and the whole book is excellent manual for many researchers in biochemistry, molecular biology, immunochemistry, biotechnology, and medicine.” (G. Wiederschain, Biochemistry, Vol. 75 (4), 2010)

Bibliographic Information

  • Book Title: Glycomics

  • Book Subtitle: Methods and Protocols

  • Editors: Niclas G. Karlsson, Nicolle H. Packer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-022-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-774-7Published: 01 April 2009

  • Softcover ISBN: 978-1-61737-769-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-022-5Published: 11 March 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 390

  • Topics: Biochemistry, general, Human Physiology, Carbohydrate Chemistry

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