Editors:

Harry Iland⁰,
Mark Hertzberg¹,
Paula Marlton²

Harry Iland
1. Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia
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Mark Hertzberg
1. Haematology Department, Westmead Hospital, Westmead, Australia
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Paula Marlton
1. Haematology Department, Princess Alexandra Hospital, Brisbane, Australia
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Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Medicine (MIMM, volume 125)

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Table of contents (18 protocols)

Front Matter

Pages i-xi

PDF
Isolation of RNA and DNA From Leukocytes and cDNA Synthesis
- Joop H. Jansen, Bert A. van der Reijden
Pages 1-11
Cytogenetic and FISH Techniques in Myeloid Malignancies
- Lynda J. Campbell
Pages 13-26
Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies
- Christophe Picard, Monique Silvy, Jean Gabert
Pages 27-68
Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR
- Susan Branford, Timothy Hughes
Pages 69-92
Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
- Susan Branford, Timothy Hughes
Pages 93-106
Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia
- Lynda J. Campbell
Pages 107-114
Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR
- Vincenzo Rossi, Laura Levati, Andrea Biondi
Pages 115-126
Diagnosis and Monitoring of PML-RARα-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR
- Elisa Mokany, Alison V. Todd, Caroline J. Fuery, Tanya L. Applegate
Pages 127-147
Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR
- Khalid Tobal, John A. Liu Yin
Pages 149-161
Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR
- Bert A. van der Reijden, Joop H. Jansen
Pages 163-175
Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia
- Jan Cools, Elizabeth H. Stover, D. Gary Gilliland
Pages 177-187
FLT3 Mutations in Acute Myeloid Leukemia
- Hitoshi Kiyoi, Tomoki Naoe
Pages 189-197
WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes
- Daniela Cilloni, Enrico Gottardi, Giuseppe Saglio
Pages 199-211
Classification of AML by DNA-Oligonucleotide Microarrays
- Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, Torsten Haferlach
Pages 213-240
Classification of AML Using a Monoclonal Antibody Microarray
- Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott et al.
Pages 241-251
Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders
- Peter J. Campbell, Linda M. Scott, Joanna E. Baxter, Anthony J. Bench, Anthony R. Green, Wendy N. Erber
Pages 253-264
Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia
- Maurizio Martini, Luciana Teofili, Luigi M. Larocca
Pages 265-273
Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation
- Thomas Lion, Franz Watzinger
Pages 275-295
Back Matter

Pages 297-306

PDF

About this book

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Keywords

Editors and Affiliations

Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia

Harry Iland
Haematology Department, Westmead Hospital, Westmead, Australia

Mark Hertzberg
Haematology Department, Princess Alexandra Hospital, Brisbane, Australia

Paula Marlton

Bibliographic Information

Book Title: Myeloid Leukemia
Book Subtitle: Methods and Protocols
Editors: Harry Iland, Mark Hertzberg, Paula Marlton
Series Title: Methods in Molecular Medicine
DOI: https://doi.org/10.1385/1597450170
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Hardcover ISBN: 978-1-58829-485-2Published: 15 November 2005
Softcover ISBN: 978-1-60761-456-2Published: 21 November 2020
eBook ISBN: 978-1-59745-017-1Published: 02 February 2008
Series ISSN: 1543-1894
Series E-ISSN: 1940-6037
Edition Number: 1
Number of Pages: XI, 307
Topics: Hematology

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Myeloid Leukemia

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Table of contents (18 protocols)

Front Matter

Isolation of RNA and DNA From Leukocytes and cDNA Synthesis

Cytogenetic and FISH Techniques in Myeloid Malignancies

Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies

Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate

Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia

Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR

Diagnosis and Monitoring of PML-RARα-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR

Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR

Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR

Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia

FLT3 Mutations in Acute Myeloid Leukemia

WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes

Classification of AML by DNA-Oligonucleotide Microarrays

Classification of AML Using a Monoclonal Antibody Microarray

Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders

Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia

Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation

Back Matter

About this book

Keywords

Editors and Affiliations

Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia

Haematology Department, Westmead Hospital, Westmead, Australia

Haematology Department, Princess Alexandra Hospital, Brisbane, Australia

Bibliographic Information

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Table of contents (18 protocols)

Front Matter

Back Matter

About this book

Keywords

Editors and Affiliations

Institute of Haematology, Royal Prince Alfred Hospital, Camperdown, Australia

Haematology Department, Westmead Hospital, Westmead, Australia

Haematology Department, Princess Alexandra Hospital, Brisbane, Australia

Bibliographic Information

Publish with us

Buy it now

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