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Epidermal Growth Factor

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Tarun B. Patel

    1. Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Maywood

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    You can also search for this editor in PubMed   Google Scholar

  2. Paul J. Bertics

    1. Department of Biomolecular Chemistry, University of Wisconsin, Madison

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 327)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. A Historical Perspective of the EGF Receptor and Related Systems

    • Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics et al.
    Pages 1-24

  3. Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography

    • Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, Paul J. Bertics
    Pages 25-38

  4. Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay

    • Jacqueline M. Lafky, Andre T. Baron, Nita J. Maihle
    Pages 39-47

  5. Analysis of EGF Receptor Interactions With the α Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs

    • Deepti Chaturvedi, Francis Edwin, Hui Sun, Tarun B. Patel
    Pages 49-59

  6. Regulator of Epidermal Growth Factor Signaling

    • Esther Sook Miin Wong, Graeme R. Guy
    Pages 61-83

  7. Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway

    • Oliver M. Fischer, Stefan Hart, Axel Ullrich
    Pages 85-97

  8. A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor

    • Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, Carl P. Blobel
    Pages 99-113

  9. In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases

    • Menachem Katz, Yaron Mosesson, Yosef Yarden
    Pages 115-129

  10. Assays to Monitor Degradation of the EGF Receptor

    • Mirko H. H. Schmidt, Ivan Dikic
    Pages 131-138

  11. Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members

    • Adriano Marchese
    Pages 139-145

  12. Epithelial Cell Migration in Response to Epidermal Growth Factor

    • Reema Zeineldin, Laurie G. Hudson
    Pages 147-158

  13. Motility Signaled From the EGF Receptor and Related Systems

    • Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith et al.
    Pages 159-177

  14. Methods for Determining the Proliferation of Cells in Response to EGFR Ligands

    • Gregory J. Wiepz, Francis Edwin, Tarun Patel, Paul J. Bertics
    Pages 179-187

  15. Clinical Advancement of EGFR Inhibitors in Cancer Therapy

    • Prakash Chinnaiyan, Paul M. Harari
    Pages 189-202

  16. Back Matter

    Pages 203-209
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Keywords

About this book

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

"The role of epidermal growth factor (EGF) in both normal development and malignancy has been elucidated gradually over the past half century … . this book could well prove a great source of help and inspiration to anyone trying to develop kits to simplify and streamline the analysis of the EGF system. … would also be a good introduction to EGF history, physiology and possible future developments, so it could be worth looking out for in the library … ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)

Editors and Affiliations

  • Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Maywood

    Tarun B. Patel

  • Department of Biomolecular Chemistry, University of Wisconsin, Madison

    Paul J. Bertics

Bibliographic Information

  • Book Title: Epidermal Growth Factor

  • Book Subtitle: Methods and Protocols

  • Editors: Tarun B. Patel, Paul J. Bertics

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159745012X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-421-0Published: 15 December 2005

  • Softcover ISBN: 978-1-61737-557-6Published: 09 December 2010

  • eBook ISBN: 978-1-59745-012-6Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 208

  • Number of Illustrations: 45 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology

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