Epidermal Growth Factor

1. Front Matter

   Pages i-xi

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2. A Historical Perspective of the EGF Receptor and Related Systems

   • Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics et al.

   Pages 1-24

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3. Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography

   • Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, Paul J. Bertics

   Pages 25-38

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4. Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay

   • Jacqueline M. Lafky, Andre T. Baron, Nita J. Maihle

   Pages 39-47

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5. Analysis of EGF Receptor Interactions With the α Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs

   • Deepti Chaturvedi, Francis Edwin, Hui Sun, Tarun B. Patel

   Pages 49-59

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6. Regulator of Epidermal Growth Factor Signaling

   • Esther Sook Miin Wong, Graeme R. Guy

   Pages 61-83

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7. Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway

   • Oliver M. Fischer, Stefan Hart, Axel Ullrich

   Pages 85-97

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8. A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor

   • Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, Carl P. Blobel

   Pages 99-113

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9. In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases

   • Menachem Katz, Yaron Mosesson, Yosef Yarden

   Pages 115-129

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10. Assays to Monitor Degradation of the EGF Receptor

    • Mirko H. H. Schmidt, Ivan Dikic

    Pages 131-138

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11. Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members

    • Adriano Marchese

    Pages 139-145

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12. Epithelial Cell Migration in Response to Epidermal Growth Factor

    • Reema Zeineldin, Laurie G. Hudson

    Pages 147-158

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13. Motility Signaled From the EGF Receptor and Related Systems

    • Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith et al.

    Pages 159-177

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14. Methods for Determining the Proliferation of Cells in Response to EGFR Ligands

    • Gregory J. Wiepz, Francis Edwin, Tarun Patel, Paul J. Bertics

    Pages 179-187

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15. Clinical Advancement of EGFR Inhibitors in Cancer Therapy

    • Prakash Chinnaiyan, Paul M. Harari

    Pages 189-202

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16. Back Matter

    Pages 203-209

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A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• Activation
• Migration
• PCR
• Vivo
• adenylyl cyclase
• molecular biology
• reproducible techniques

From the reviews:

"The role of epidermal growth factor (EGF) in both normal development and malignancy has been elucidated gradually over the past half century … . this book could well prove a great source of help and inspiration to anyone trying to develop kits to simplify and streamline the analysis of the EGF system. … would also be a good introduction to EGF history, physiology and possible future developments, so it could be worth looking out for in the library … ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)

• Department of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Maywood

  Tarun B. Patel

• Department of Biomolecular Chemistry, University of Wisconsin, Madison

  Paul J. Bertics

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