Food-Borne Pathogens

1. Front Matter

   Pages i-xi

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2. The Bacteria

   1. Front Matter

      Pages 1-1

      PDF

   2. Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources

      • Rosabel Falcón, Tatiana d’Albuquerque, Maria das Graças de Luna, Angela Corrêa de Freitas-Almeida, Tomomasa Yano

      Pages 3-13

   3. Detection and Purification of Bacillus cereus Enterotoxins

      • Toril Lindbäck, Per Einar Granum

      Pages 15-26

   4. Campylobacter

      • Rachel Gorman, Catherine C. Adley

      Pages 27-35

   5. Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces

      • Miia Lindström, Mari Nevas, Hannu Korkeala

      Pages 37-45

   6. Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex

      • Peter C. H. Feng, Steven R. Monday

      Pages 47-55

   7. PulseNet’s Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis

      • Lewis M. Graves, Balasubramanian Swaminathan

      Pages 57-72

   8. Plesiomonas shigelloides

      • Ivan Ciznar, Carlos González-Rey, Karel Krovacek, Anna Hostacka

      Pages 73-80

   9. Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies

      • Rachel Gorman, Catherine C. Adley

      Pages 81-90

   10. Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus

       • Moira M. Brett

       Pages 91-98

   11. Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples

       • Keya De, Ranjan K. Nandy, G. Balakrish Nair

       Pages 99-124

   12. HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes

       • Jennifer Colford

       Pages 125-131

3. The Viruses

   1. Front Matter

      Pages 133-133

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   2. Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR

      • Christian M. Beuret

      Pages 135-152

   3. Detection of Enteroviruses

      • Miguel-Angel Jiménez-Clavero, Victoria Ley, Nuria Gómez, Juan-Carlos Sáiz

      Pages 153-169

   4. Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

      • Julie Jean, Ismaïl Fliss

      Pages 171-187

4. The Parasites

   1. Front Matter

      Pages 189-189

      PDF

   2. Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis

      • Sandra M. O’Neill, Grace Mulcahy, John P. Dalton

      Pages 191-201

   3. Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish

      • Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, John E. Moore

      Pages 203-215

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• Aflatoxin
• Lysin
• Pathogen
• Polymerasekettenreaktion
• Salmonella
• Seafood
• Toxin
• biotechnology
• virus

• Microbiology Laboratory Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland

  Catherine C. Adley

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