Placenta and Trophoblast

1. Front Matter

   Pages i-xix

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2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. Overview I

      • Michael J. Soares, Joan S. Hunt

      Pages 3-6

3. Methods for Studying Embryo Implantation and Uterine Biology

   1. Front Matter

      Pages 7-7

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   2. Methodologies to Study Implantation in Mice

      • Kaushik Deb, Jeff Reese, Bibhash C. Paria

      Pages 9-34

   3. Blastocyst Culture

      • D. Randall Armant

      Pages 35-56

   4. Isolation of Hormone Responsive Uterine Stromal Cells

      • Virginia Rider

      Pages 57-67

   5. Rat Decidual Cell Cultures

      • Yan Gu, Geula Gibori

      Pages 69-78

   6. The Immortalization of Human Endometrial Cells

      • Graciela Krikun, Gil Mor, Charles Lockwood

      Pages 79-83

   7. Sheep Uterine Gland Knockout (UGKO) Model

      • Thomas E. Spencer, C. Allison Gray

      Pages 85-94

   8. A Baboon Model for Inducing Endometriosis

      • Asgerally T. Fazleabas

      Pages 95-99

   9. A Baboon Model for Simulating Pregnancy

      • Asgerally T. Fazleabas

      Pages 101-110

   10. The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research

       • Almuth Einspanier, Kai Lieder, Ralf Einspanier, Bettina Husen

       Pages 111-121

4. In Vitro Trophoblast and Placental Model Systems

   1. Front Matter

      Pages 123-123

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   2. Mouse Trophoblast Stem Cells

      • Jennifer Quinn, Tilo Kunath, Janet Rossant

      Pages 125-148

   3. Connexins and Trophoblast Cell Lineage Development

      • Mark Kibschull, Elke Winterhager

      Pages 149-158

   4. Rcho-1 Trophoblast Stem Cells

      • Namita Sahgal, Lindsey N. Canham, Brent Canham, Michael J. Soares

      Pages 159-178

   5. Bovine Trophoblast Cell Culture Systems

      • Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, Toru Takahashi

      Pages 179-188

   6. In Vitro Induction of Trophoblast from Human Embryonic Stem Cells

      • Ren-He Xu

      Pages 189-202

   7. Isolation and Culture of Term Human Trophoblast Cells

      • Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, Joan S. Hunt

      Pages 203-217

A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

• Implantat
• cells
• development
• electron microscope
• microscope

"...provides step-by-step instructions for conducting research in mammalian embryology." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

 

"...an important contribution to the literature...recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal

• Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City

  Michael J. Soares

• Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City

  Joan S. Hunt

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