Human Papillomaviruses

1. Front Matter

   Pages i-xv

   PDF

2. Identification of New Papillomavirus Types

   • Ethel-Michele de Villiers, Corinna Whitley, Karin Gunst

   Pages 1-13

3. Identification of HPV Variants

   • John Cason, Jon Bible, Christine Mant

   Pages 15-25

4. Histochemical Analysis of Cutaneous HPV-Associated Lesions

   • Kiyofumi Egawa

   Pages 27-40

5. Histological Analysis of Cervical Intraepithelial Neoplasia

   • Michael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, David Jenkins

   Pages 41-48

6. Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections

   • Woei Ling Peh, John Doorbar

   Pages 49-59

7. Detection and Quantitation of HPV Gene Expression Using Real-Time PCR

   • Rashmi Seth, John Rippin, Li Guo, David Jenkins

   Pages 61-72

8. Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers

   • Svetlana Vinokurova, Nicolas Wentzensen, Magnus von Knebel Doeberitz

   Pages 73-83

9. Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN

   • Jan P. A. Baak, Arnold-Jan Kruse

   Pages 85-99

10. HPV DNA Detection and Typing in Cervical Scrapes

    • Peter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, René P. Pol, Chris J. L. M. Meijer

    Pages 101-114

11. HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions

    • Maurits de Koning, Linda Struijk, Mariet Feltkamp, Jan ter Schegget

    Pages 115-127

12. Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies

    • Margaret Anne Stanley

    Pages 129-139

13. Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures

    • Paul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, Tomomi Nakahara

    Pages 141-155

14. Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose

    • Regina Wilson, Laimonis A. Laimins

    Pages 157-169

15. Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture

    • Margaret E. McLaughlin-Drubin, Craig Meyers

    Pages 171-186

16. Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic “Raft” Cultures

    • N. Sanjib Banerjee, Louise T. Chow, Thomas R. Broker

    Pages 187-202

17. The HPV Xenograft Severe Combined Immunodeficiency Mouse Model

    • William Bonnez

    Pages 203-216

18. The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease

    • Janet L. Brandsma

    Pages 217-235

19. Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology

    • Yong Liu, Hong You, Paul L. Hermonat

    Pages 237-245

20. Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae

    • Peter C. Angeletti

    Pages 247-260

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• DNA
• PCR
• Southern Blot
• Virus
• cell lines
• gene expression
• genes
• infection
• infections
• infectious
• saccharomyces cerevisiae
• transcription
• infectious diseases

• Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK

  John Doorbar

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