Breast Cancer Research Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Preparation of Cell and Tissue Samples

   1. Front Matter

      Pages 1-1

      PDF

   2. Breast Tissue Banking

      • Linda Snell, Peter H. Watson

      Pages 3-24

   3. Frozen Biopsy Collection and Storage

      • Russell Leek

      Pages 25-28

   4. Serial Biopsies/Fine-Needle Aspirates and Their Assessment

      • Irene Boeddinghaus, Stephen R. D. Johnson

      Pages 29-41

   5. Breast Tissue Microarrays

      • Robert J. Springall, ChlllLeryl E. Gillett

      Pages 43-50

   6. Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA

      • Naomi Robertson, Russell Leek

      Pages 51-53

   7. Isolation of RNA From Tumor Samples

      • Naomi Robertson, Russell Leek

      Pages 55-59

   8. Isolation of DNA From Tumor Samples

      • Naomi Robertson, Russell Leek

      Pages 61-63

   9. Laser-Assisted Microdissection and Isolation of DNA and RNA

      • Ulrich Lehmann, Hans Kreipe

      Pages 65-75

3. Markers of Clinical Outcome and Prognosis

   1. Front Matter

      Pages 77-77

      PDF

   2. Traditional and Established Indicators of Prognosis and Treatment Success

      • Derek E. Roskell, Ian D. Buley

      Pages 79-89

   3. Sentinel Lymph Node Biopsy in Breast Cancer

      • Celia Chao, Kelly M. McMasters

      Pages 91-111

   4. Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer

      • Michael Z. Gilcrease, Aysegul Sahin

      Pages 113-125

   5. Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections

      • Bharat Jasani, Anthony Douglas-Jones, Anthony Rhodes, Susan Wozniak, Peter J. Barrett-Lee, Julie Gee et al.

      Pages 127-146

   6. Markers of Apoptosis

      • Fazlul H. Sarkar, Yiwei Li

      Pages 147-160

   7. Quantitative Angiogenesis in Breast Cancer

      • Stephen B. Fox

      Pages 161-187

4. Analysis of Tumor-Derived Proteins and Antigens

   1. Front Matter

      Pages 189-189

      PDF

   2. Immunohistochemistry

      • Cheryl E. Gillett

      Pages 191-200

   3. Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry

      • Tracey M. Carter, Susan A. Brooks

      Pages 201-216

A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.

• Biopsie
• Endoplasmatisches Reticulum
• Migration
• Proteomics
• Vivo
• cells
• glycoprotein
• proteins
• tissue

• School of Biological and Molecular Sciences, Oxford Brookes University, Headington, UK

  Susan A. Brooks

• Medical Oncology Unit, The Churchill Hospital, Headington, UK

  Adrian Harris

Policies and ethics