Mast Cells

1. Front Matter

   Pages i-xvii

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2. Overview of Mast Cell Biology

   1. Front Matter

      Pages 1-1

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   2. Paul Ehrlich’’s “;Mastzellen”— From Aniline Dyes to DNA Chip Arrays

      • Harsha Vyas, Guha Krishnaswamy

      Pages 3-11

   3. The Human Mast Cell

      • Guha Krishnaswamy, Omar Ajitawi, David S. Chi

      Pages 13-34

   4. Mast Cells in Allergy and Autoimmunity

      • Gregory D. Gregory, Melissa A. Brown

      Pages 35-50

3. Identification of Mast Cells in Culture and in Tissue

   1. Front Matter

      Pages 51-51

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   2. Analysis of MCT and MCTC Mast Cells in Tissue

      • Lawrence B. Schwartz

      Pages 53-62

   3. Mast Cell Ultrastructure and Staining in Tissue

      • Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, George A. Youngberg

      Pages 63-76

   4. Expression of Cell Surface Antigens on Mast Cells

      • Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr et al.

      Pages 77-90

   5. Identification of Mast Cells in the Cellular Response to Myocardial Infarction

      • Nikolaos G. Frangogiannis, Mark L. Entman

      Pages 91-101

4. Development of Mast Cells In Vitro

   1. Front Matter

      Pages 103-103

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   2. Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells

      • Arnold S. Kirshenbaum, Dean D. Metcalfe

      Pages 105-112

   3. Culture of Human Mast Cells From Hemopoietic Progenitors

      • Hirohisa Saito

      Pages 113-122

   4. Isolation, Culture, and Characterization of Intestinal Mast Cells

      • Gernot Sellge, Stephan C. Bischoff

      Pages 123-138

5. Mast Cell Signaling and Gene Expression

   1. Front Matter

      Pages 139-139

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   2. Activation of Nuclear Factor-κB

      • Chuanfu Li, Jim Kelley, Tuanzhu Ha

      Pages 141-150

   3. Analysis of Mitogen-Activated Protein Kinase Activation

      • Stephen C. Armstrong

      Pages 151-163

   4. Microarray and Gene-Clustering Analysis

      • Tsung-Hsien Tsai, Denise M. Milhorn, Shau-Ku Huang

      Pages 165-174

   5. Techniques to Study FcεRI Signaling

      • Yuko Kawakami, Jiro Kitaura, Toshiaki Kawakami

      Pages 175-189

6. Mast Cell Expression and Release of Inflammatory Mediators of Inflammatory Mediators

   1. Front Matter

      Pages 191-191

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A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.

• Antigen
• DNA
• RNA
• biology
• cell biology
• cytokine
• development
• gene expression
• genes
• microarray
• molecular biology
• protein
• regulation
• reproducible techniques
• tissue

From the reviews:

"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. … The information will be of value to advanced students and laboratory investigators. … Each of the substantive chapters contains detailed protocols and a short set of primary references. … there is sufficient detail to allow direct adaptation in the laboratory. … the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody’s Book Review Service, March, 2006)

• Quillen College of Medicine, James H. Quillen Veterans Affairs Medical Center, East Tennessee State University, Johnson City

  Guha Krishnaswamy, David S. Chi

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