Chemical Genomics

1. Front Matter

   Pages i-xi

   PDF

2. Reviews

   1. Front Matter

      Pages 1-1

      PDF

   2. Introduction to Chemical Genomics

      • Paul R. Caron

      Pages 3-10

   3. Chemistry for Chemical Genomics

      • Lutz Weber

      Pages 11-24

   4. Computer-Aided Design of Small Molecules for Chemical Genomics

      • Philip M. Dean

      Pages 25-39

3. Protocols

   1. Front Matter

      Pages 41-41

      PDF

   2. Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography

      • Ana Cecília A. Roque, Geeta Gupta, Christopher R. Lowe

      Pages 43-62

   3. The Role and Application of In Silico Docking in Chemical Genomics Research

      • Aldo Jongejan, Chris de Graaf, Nico P. E. Vermeulen, Rob Leurs, Iwan J. P. de Esch

      Pages 63-91

   4. Synthesis of Complex Carbohydrates and Glyconjugates

      • Kang Ryu, Steven Lin, Jun Shao, Jing Song, Min Chen, Wei Wang et al.

      Pages 93-105

   5. High-Throughput Cloning for Proteomics Research

      • Sharon A. Doyle

      Pages 107-113

   6. Screening for the Expression of Soluble Recombinant Protein in Escherichia coli

      • Sharon A. Doyle

      Pages 115-121

   7. High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli

      • Michael B. Murphy, Sharon A. Doyle

      Pages 123-130

   8. The Wheat Germ Cell-Free Expression System

      • Tatsuya Sawasaki, Mudeppa D. Gouda, Takayasu Kawasaki, Takafumi Tsuboi, Yuzuru Toscana, Kazuyuki Takai et al.

      Pages 131-144

   9. Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System

      • Yaeta Endo, Tatsuya Sawasaki

      Pages 145-167

   10. Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System

       • Toshiyuki Kohno

       Pages 169-185

   11. Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening

       • Jon Hoyt, Randall W. King

       Pages 187-195

   12. Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis

       • Jonathan M. Blackburn, Darren J. Hart

       Pages 197-216

   13. Peptide and Small-Molecule Microarrays

       • Jan Marik, Kit S. Lam

       Pages 217-226

   14. Peptide Mass Fingerprinting

       • Judith Webster, David Oxley

       Pages 227-240

   15. A Practical Protocol for Carbohydrate Microarrays

       • Ruobing Wang, Shaoyi Liu, Dhaval Shah, Denong Wang

       Pages 241-252

Chemical genomics is an exciting new field that aims to transform biolo- cal chemistry into a high-throughput industrialized process, much in the same way that molecular biology has been transformed by genomics. The inter- tion of small organic molecules with biological systems (mostly proteins) underpins drug discovery in the pharmaceutical and biotechnology industries, and therefore a volume of laboratory protocols that covers the key aspects of chemical genomics would be of use to biologists and chemists in these orga- zations. Academic scientists have been exploring the functions of proteins using small molecules as probes for many years and therefore would also b- efit from sharing ideas and laboratory procedures. Whatever the organizational backgrounds of the scientists involved, the challenges of extracting the ma- mum human benefit from genome sequencing projects remains considerable, and one where it is increasingly recognized that chemical genomics will play an important part. Chemical Genomics: Reviews and Protocols is divided into two sections, the first being a series of reviews to describe what chemical genomics is about and to set the scene for the protocol chapters. The subject is introduced by Paul Caron, who explains the various flavors of chemical genomics. This is f- lowed by Lutz Weber and Philip Dean who cover the interaction between organic molecules and protein targets from the different perspectives of la- ratory experimentation and in silico design. The protocols begin with the me- ods developed in Christopher Lowes’ laboratory (Roque et al.

• Escherichia coli
• Microarray
• Proteomics
• genetic information
• molecular biology
• organic chemistry
• proteins
• reproducible techniques

"...an elaborate combination of scientific background information with well-illustrated  protocols...a prime reference book for scientists." -ChemBioChem

• CamBP Ltd., Cambridge, UK

  Edward D. Zanders

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