Interferon Methods and Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Biological and Clinical Basis for Molecular Studies of Interferons

   • Katie R. Pang, Jashin J. Wu, David B. Huang, Stephen K. Tyring, Samuel Baron

   Pages 1-23

3. Interferon Research

   • Myriam S. Kunzi, Paula M. Pitha

   Pages 25-35

4. Virus Infection and the Interferon Response

   • Marcus J. Korth, John C. Kash, Jeffrey C. Furlong, Michael G. Katze

   Pages 37-55

5. Genomic DNA Affinity Chromatography

   • Jyothi Kumaran, Eleanor N. Fish

   Pages 57-67

6. Protein Engineering of Interferon Alphas

   • Renqiu Hu, Ke-jian Lei, Joseph Bekisz, Kathryn C. Zoon

   Pages 69-80

7. Assays for the Interferon-Induced Enzyme 2′,5′ Oligoadenylate Synthetases

   • Saumendra N. Sarkar, Mitali Pandey, Ganes C. Sen

   Pages 81-101

8. A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2′,5′ Oligoadenylates

   • Chandar S. Thakur, Zan Xu, Zhengfu Wang, Zachary Novince, Robert H. Silverman

   Pages 103-113

9. Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assay

   • Shalina S. Ousman, Iain L. Campbell

   Pages 115-134

10. Mitogen-Activated Protein Kinase Pathways in Interferon Signaling

    • Antonella Sassano, Amit Verma, Leonidas C. Platanias

    Pages 135-150

11. Development of an Interferon-Based Cancer Vaccine Protocol

    • W. Robert Fleischmann Jr., Tzu G. Wu

    Pages 151-166

12. Type I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells

    • Stefano M. Santini, Caterina Lapenta, Filippo Belardelli

    Pages 167-181

13. Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations

    • Stacey L. Olshalsky, Patricia Fitzgerald-Bocarsly

    Pages 183-194

14. Analysis of Anti-Interferon Properties of the Herpes Simplex Virus Type I ICP0 Protein

    • Karen Mossman

    Pages 195-205

15. Interferon Subtype Gene Therapy for Regulating Cytomegalovirus Disease

    • Cassandra M. James, Emmalene J. Bartlett, Josephine P. Mansfield, Vanessa S. Cull

    Pages 207-219

16. Transfection of Müller Cells With Type I Interferon Transgenes

    • Benitta John-Philip, Daniel J. J. Carr

    Pages 221-232

17. Back Matter

    Pages 233-236

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A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.

• cancer
• gene therapy
• infection
• interferon
• research
• resistance
• vaccine
• virus

• Department of Ophthalmology, The University of Oklahoma Health and Sciences Center, Oklahoma City

  Daniel J. J. Carr

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