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  • © 2005

Molecular Diagnostics

For the Clinical Laboratorian

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Table of contents (44 chapters)

  1. Front Matter

    Pages i-xvi
  2. Introduction

    1. Front Matter

      Pages 1-1
  3. Basic Molecular Biology

    1. Front Matter

      Pages 11-11
    2. Extraction of Nucleic Acids

      • Paul N. Bogner, Anthony A. Killeen
      Pages 25-30
    3. Nucleic Acid Blotting Techniques

      • Terry Amiss, Sharon Collins Presnell
      Pages 31-46
    4. The Polymerase Chain Reaction

      • William B. Coleman, Gregory J. Tsongalis
      Pages 47-55
    5. Bioinformatics

      • Sharon L. Ricketts
      Pages 57-61
  4. Molecular Diagnostic Technologies

    1. Front Matter

      Pages 63-63
    2. PCR-Based Methods for Mutation Detection

      • Ian M. Frayling, Emma Monk, Rachel Butler
      Pages 65-74
    3. Alternative Methods for Amplified Nucleic Acid Testing

      • Deborah A. Payne, Laurie E. Sower
      Pages 75-84
    4. Single-Nucleotide Polymorphisms

      • Ulrich Broeckel, Martin J. Hessner
      Pages 111-120
    5. Microarray Approaches to Gene Expression Analysis

      • David Neil Hayes, Matthew Meyerson
      Pages 121-148
    6. Methods for Analysis of DNA Methylation

      • Alexander Dobrovic
      Pages 149-160
  5. Other Clinical Diagnostic Technologies

    1. Front Matter

      Pages 161-161
    2. Flow Cytometry

      • Joseph A. DiGiuseppe
      Pages 163-172
    3. Medical Cytogenetics

      • Martha B. Keagle
      Pages 173-187
    4. Fluorescence In Situ Hybridization

      • Suneel D. Mundle, Robert J. Koska
      Pages 189-202

About this book

Taking advantage of the many major advances that have occurred since their groundbreaking first edition was published, William B. Coleman and Gregory J. Tsongalis have updated and expanded their highly praised tutorial guide to molecular diagnostic techniques to include not only improved traditional methods, but also totally new molecular technologies, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that are complementary to molecular diagnostic methodologies, and also discuss genetic counseling and the ethical and social issues involved with nucleic acid testing.

Authoritative and state-of-the-art, Molecular Diagnostics: For the Clinical Laboratorian, 2nd Edition is the essential textbook of choice for anyone working in molecular diagnostics and who wants to remain current with this rapidly changing field.

Reviews

"This is a great book to have handy for ready reference in this rapidly evolving field. While the reader may not use this reference daily, there is no question that it will be needed for those occasions when such testing is ordered and a clinical consultation between the clinical laboratory and the healthcare provider is necessary." - Doody's Health Sciences Book Review Journal

"...the textbook of choice for anyone working in molecular diagnostics who wants to remain current with this rapidly changing field. -Clinical Laboratory International

From Reviews of the First Edition...

"...a very good introduction to the basic elements of molecular pathology...a clear, enjoyable read...an excellent discussion of methods." -Clinical Chemistry

"...highly recommended...for those interested in clinical molecular diagnosis...will satisfy those who are more expert in certain areas of molecular diagnosis, but who have been unable to keep up with the wide range of recent advances in molecular diagnosis." -American Journal of Clinical Pathology

"...gives a good theoretical background for those with little experience in this field. It is readable, comprehensive, and...up-to-date." -Journal of Clinical Pathology

"...well written, comprehensive...should bring even the reader with limited background knowledge up to speed on what is a rapidly progressing field." -Trends in Molecular Medicine

"...a timely and highly relevant book that will serve as a valuable resource to those practicing molecular diagnostics from the bench, as well as to basic and clinical scientists..." -Endocrine News

Editors and Affiliations

  • Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, USA

    William B. Coleman

  • Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, USA

    Gregory J. Tsongalis

Bibliographic Information

  • Book Title: Molecular Diagnostics

  • Book Subtitle: For the Clinical Laboratorian

  • Editors: William B. Coleman, Gregory J. Tsongalis

  • DOI: https://doi.org/10.1385/1592599281

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Medicine, Medicine (R0)

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-356-5Published: 15 August 2005

  • eBook ISBN: 978-1-59259-928-8Published: 28 October 2007

  • Edition Number: 2

  • Number of Pages: XVIII, 567

  • Topics: Pathology, Laboratory Medicine, Human Genetics, Microbiology

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access