Microarrays in Clinical Diagnostics

1. Front Matter

   Pages i-xiv

   PDF

2. Noncontact Laser Microdissection and Pressure Catapulting

   • Yilmaz Niyaz, Monika Stich, Bernd Sägmüller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer et al.

   Pages 1-24

3. Applications of the Universal DNA Microarray in Molecular Medicine

   • Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, Francis Barany

   Pages 25-58

4. Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization

   • Zhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li et al.

   Pages 59-78

5. Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays

   • Lovisa Lovmar, Ann-Christine Syvänen

   Pages 79-92

6. Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology

   • Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, Stefania Stenirri

   Pages 93-106

7. Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader®

   • Margaret A. Keller

   Pages 107-119

8. Controlled Agitation During Hybridization

   • Achim Wixforth

   Pages 121-145

9. Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Conductance Regulator Gene by Luminex® xMAP™ Suspension Array

   • Sherry A. Dunbar, James W. Jacobson

   Pages 147-171

10. Protein Microarray-Based Screening of Antibody Specificity

    • Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, Paul F. Predki

    Pages 173-182

11. Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection

    • Brian B. Haab

    Pages 183-194

12. Allergen Microarrays

    • Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, Andrea Crisanti

    Pages 195-207

13. Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples

    • Ruochun Huang, Qian Shi, Weimin Yang, Ruo-Pan Huang

    Pages 209-221

14. Multiplexed Cytokine Sandwich Immunoassays Clinical Applications

    • Uma Prabhakar, Edward Eirikis, Bruce E. Miller, Hugh M. Davis

    Pages 223-232

15. Validation and Quality Control of Protein Microarray-Based Analytical Methods

    • Larry J. Kricka, Stephen R. Master

    Pages 233-255

16. Tissue Microarrays

    • Ronald Simon, Martina Mirlacher, Guido Sauter

    Pages 257-268

17. Back Matter

    Pages 269-274

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Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• DNA
• PCR
• SNP
• diagnostics
• genes
• hybridization

From the reviews:

"a volume for the specialist with a fascination for the optimal collection and analysis of large data sets in microarray format."-SGM Quarterly

"...there is no other comparable book on the market...highly recommended to anyone interested in this rapidly evolving field of technology..." - Doody's Health Science Book Review Journal

"Microarray technology has become a mainstay of biological research. … The book is divided into 15 chapters … . Overall this book offers a good read to both clinicians and researchers at the bench who wish to enter the field of microarrays. The format of the book also provides a good roadmap to graduate students wishing to translate aspects of their research to methods amenable to microarrays." (Sridar V. Chittur, Clinical Chemistry, Vol. 52 (6), June, 2006)

"Microarrays in Clinical Diagnostics … is a collection of fifteen chapters by authors from both industry and academia, surveying current applications of microarray-based technologies in diagnostics … . provides protocols in the successful style of the Methods in Molecular Biology series, with clarity and sufficient technical detail so that both novice and current microarray users are sure to benefit from this book." (Nicolas Winssinger, ChemMedChem, Issue 1, 2006)

• Biochemistry Department, NMI Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany

  Thomas O. Joos

• Center for Translational Medicine, Department of Medicine, Jefferson Medical College, Philadelphia

  Paolo Fortina

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