Molecular Cardiology

1. Front Matter

   Pages i-xv

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2. Introduction

   1. Front Matter

      Pages i-xv

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   2. Introduction to Methods in Molecular Cardiology

      • Zhongjie Sun

      Pages 3-12

3. Gene Transfer and Gene Therapy for Cardiovascular Disease

   1. Front Matter

      Pages 13-13

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   2. Gene Therapy Approaches to Cardiovascular Disease

      • J. Mark Jones, Walter J.Koch

      Pages 15-35

   3. Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System

      • Yi Tang

      Pages 37-47

   4. Cardiac Gene Delivery Using DNA–Adenoviral Constructs

      • J. Mark Jones, Walter J. Koch

      Pages 49-57

   5. Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology

      • Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, Kaushik P. Patel

      Pages 59-79

   6. TAT-Mediated Protein Transduction

      • Åsa B. Gustafsson, Roberta A. Gottlieb, David J. Granville

      Pages 81-90

   7. Expression of Human eNOS in Cardiac and Endothelial Cells

      • Xiuqing Wang, Robert Cade, Zhongjie Sun

      Pages 91-107

   8. Strategies of Conditional Gene Expression in Myocardium

      • Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, Thomas J. Podor

      Pages 109-154

   9. Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes

      • Hideko Kasahara, Hiroki Aoki

      Pages 155-172

4. Stem Cell Therapy for Cardiovascular Disease

   1. Front Matter

      Pages 173-173

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   2. Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair

      • Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Mènard, Michel Puc

      Pages 175-182

   3. Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair

      • Yao Liang Tang

      Pages 183-192

   4. Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure

      • Yao Liang Tang

      Pages 193-204

   5. Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes

      • Sharon Etzion, Radka Holbova, Liron Miller, Jonathan Leor

      Pages 205-221

   6. Methods for Examining Stem Cells in Post-Ischemic and Transplanted Hearts

      • Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus et al.

      Pages 223-238

   7. Endothelial Progenitor Cell Culture and Gene Transfer

      • Hideki Iwaguro, Takayuki Asahara

      Pages 239-247

5. Gene Analysis in the Injured and Hypertrophied Heart

   1. Front Matter

      Pages 249-249

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The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models of heart diseases (cardiac hypertrophy, cardiac dysfunction, and so on. ) are powerful tools for the study of heart disease pathogenesis. Methods for gene transfer to heart tissue using viral and nonviral vectors form the basis of gene therapy for heart diseases. Heart-specific promoters containing a hypox- inducible cardioprotective gene switch are key for protection of the heart from ischemia. Gene and stem cell therapies open novel and exciting avenues for the prevention and treatment of heart diseases. Molecular Cardiology: Methods and Protocols consists of 26 chapters de- ing with various aspects of molecular cardiology, including gene transfer and gene therapy for cardiovascular disease, stem cell therapy for cardiovascular disease, gene analysis in the injured and hypertrophied heart, and transgenesis in cardiovascular research. This book provides step-by-step methods for the successful completion of experimental procedures, and would be useful for both experienced and new investigators in the field of molecular cardiology.

• cardiology
• cardiovascular
• gene therapy
• heart
• heart disease
• heart failure
• myocardial infarction
• vascular disease

• Departments of Medicine and Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville

  Zhongjie Sun

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