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Medical BioMethods Handbook

  • Book
  • © 2005

Overview

Editors:
  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Ralph Rapley

    1. University of Hertfordshire, Hatfield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (44 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Basic Techniques in Molecular Biology

    • Ralph Rapley
    Pages 1-12

  3. Probe Design, Production, and Applications

    • Marilena Aquino de Muro
    Pages 13-23

  4. Restriction Enzymes

    • Gareth J. S. Jenkins
    Pages 25-34

  5. Southern Blotting as a Diagnostic Method

    • Bronwen Harvey, Pirkko Soundy
    Pages 35-42

  6. Western Blotting as a Diagnostic Method

    • Pirkko Soundy, Bronwen Harvey
    Pages 43-62

  7. Principles and Medical Applications of the Polymerase Chain Reaction

    • Bimal D. M. Theophilus
    Pages 63-72

  8. Single-Strand Conformation Polymorphism (SSCP) Analysis

    • Igor Vorechovsky
    Pages 73-77

  9. Denaturing Gradient Gel Electrophoresis (DGGE)

    • Jeroen H. Roelfsema, Dorien J. M. Peters
    Pages 79-86

  10. Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing

    • Anu Suomalainen, Ann-Christine Syvänen
    Pages 87-94

  11. Introduction to Capillary Electrophoresis of DNA

    • Beatriz Sanchez-Vega
    Pages 95-116

  12. Mapping Techniques

    • Simon G. Gregory
    Pages 117-133

  13. Dideoxyfingerprinting for Mutation Detection

    • Ioannis Bossis, Antonios Voutetakis, Constantine A. Stratakis
    Pages 135-145

  14. Conformation-Sensitive Gel Electrophoresis

    • Marian Hill
    Pages 147-153

  15. Amplification Refractory Mutation System and Molecular Diagnostics

    • Richard Kitching, Arun Seth
    Pages 155-165

  16. Ligase Chain Reaction

    • Carla Osiowy
    Pages 167-181

  17. Chemical Cleavage of Mismatch

    • Neil V. Whittock, Louise Izatt
    Pages 183-194

  18. The Protein Truncation Test

    • Johan T. den Dunnen
    Pages 195-204

  19. Linkage, Allele Sharing, and Association

    • Mara Giordano
    Pages 205-226

  20. Single-Nucleotide Polymorphisms

    • Cyril Mamotte, Frank Christiansen, Lyle J. Palmer
    Pages 227-254
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Keywords

About this book

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.        

Reviews

"...the book is a good reference work in any clinical diagnostic lab...good value for the money." - Microbiology Today

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker, Ralph Rapley

Bibliographic Information

  • Book Title: Medical BioMethods Handbook

  • Editors: John M. Walker, Ralph Rapley

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1385/1592598706

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2005

  • Hardcover ISBN: 978-1-58829-288-9Published: 18 March 2005

  • Softcover ISBN: 978-1-58829-334-3Published: 18 March 2005

  • eBook ISBN: 978-1-59259-870-0Published: 09 October 2007

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XVI, 644

  • Topics: Cell Biology

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