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Chemosensitivity

Volume I: In Vitro Assays

  • Book
  • © 2005

Overview

Editors:
  1. Rosalyn D. Blumenthal

    1. Garden State Cancer Center, Belleville

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 110)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. An Overview of Chemosensitivity Testing

    • Rosalyn D. Blumenthal
    Pages 3-18

  3. Clonogenic Cell Survival Assay

    • Anupama Munshi, Marvette Hobbs, Raymond E. Meyn
    Pages 21-28

  4. High-Sensitivity Cytotoxicity Assays for Nonadherent Cells

    • M. Jules Mattes
    Pages 29-37

  5. Sulforhodamine B Assay and Chemosensitivity

    • Wieland Voigt
    Pages 39-48

  6. Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity

    • Gertjan J. L. Kaspers
    Pages 49-57

  7. Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity

    • Hisayuki Kobayashi
    Pages 59-67

  8. The MTT Assay to Evaluate Chemosensitivity

    • Jack D. Burton
    Pages 69-78

  9. Histoculture Drug Response Assay to Monitor Chemoresponse

    • Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, Shiro Nozawa
    Pages 79-86

  10. In Vitro Testing of Chemosensitivity in Physiological Hypoxia

    • Rita Grigoryan, Nino Keshelava, Clarke Anderson, C. Patrick Reynolds
    Pages 87-100

  11. Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements

    • Christian M. Kurbacher, Ian A. Cree
    Pages 101-120

  12. High-Throughput Technology

    • Marylène Fortin, Ann-Muriel Steff, Patrice Hugo
    Pages 121-137

  13. Dimscan

    • Nino Keshelava, Tomás Frgala, Jirí Krejsa, Ondrej Kalous, C. Patrick Reynolds
    Pages 139-153

  14. The ChemoFx® Assay

    • Robert L. Ochs, Dennis Burholt, Paul Kornblith
    Pages 155-172

  15. Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models

    • C. Patrick Reynolds, Barry J. Maurer
    Pages 173-183

  16. Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity

    • John F.Gibbs, Youcef M.Rustum, Harry K.Slocum
    Pages 185-195

  17. Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing

    • Uriel Bachrach
    Pages 197-211

  18. Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity

    • Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, Seiji Kawasaki
    Pages 213-227

  19. Back Matter

    Pages 229-231
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Keywords

About this book

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.

Editors and Affiliations

  • Garden State Cancer Center, Belleville

    Rosalyn D. Blumenthal

Bibliographic Information

  • Book Title: Chemosensitivity

  • Book Subtitle: Volume I: In Vitro Assays

  • Editors: Rosalyn D. Blumenthal

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598692

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-345-9Published: 14 February 2005

  • Softcover ISBN: 978-1-61737-508-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-869-4Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 231

  • Topics: Cancer Research

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