Skip to main content
SpringerLink
Log in

Hypertension

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Jérôme P. Fennell

    1. Moyne Institute for Preventive Medicine, Trinity College, Dublin, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Andrew H. Baker

    1. Division of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 108)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Models Of Hypertension

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Congenic/Consomic Models of Hypertension

      • Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, Anna F. Dominiczak
      Pages 3-15

    3. Mouse Knockout Models of Hypertension

      • Michael Bader
      Pages 17-32

    4. Production of Transgenic Models in Hypertension

      • Elena Popova, Michael Bader, Alexander Krivokharchenko
      Pages 33-50

    5. Measuring Blood Pressure in Small Laboratory Animals

      • Klaas Kramer, René Remie
      Pages 51-62

  3. Assessment Of Free Radicals In Endothelial Dysfunction

    1. Front Matter

      Pages 63-63
      Download chapter PDF

    2. Analysis of Superoxide Anion Production in Tissue

      • Michela Zanetti, Livius V. d’Uscio, Timothy E. Peterson, Zvonimir S. Katusic, Timothy O’Brien
      Pages 65-72

    3. Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence

      • Tomasz J. Guzik, Keith M. Channon
      Pages 73-89

    4. A Guide to Wire Myography

      • Angela Spiers, Neal Padmanabhan
      Pages 91-104

  4. Nucleic Acid Techniques

    1. Front Matter

      Pages 105-105
      Download chapter PDF

    2. Selection of Candidate Genes in Hypertension

      • Charles A.
      Pages 107-129

    3. Genome-Wide Scanning With SSLPs in the Rat

      • Carol Moreno, Kathleen Kennedy, Jaime Wendt Andrae, Howard J. Jacob
      Pages 131-138

    4. Extraction of RNA From Cells and Tissue

      • Ian Bird M.
      Pages 139-148

    5. Single-Strand Conformational Polymorphism Analysis

      • Haidong Zhu
      Pages 149-158

    6. Single-Nucleotide Polymorphism Genotyping for Disease Association Studies

      • Peter Doris A.
      Pages 159-172

    7. Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System

      • Elizabeth Donohoe
      Pages 173-187

    8. TaqMan Real-Time PCR Quantification Conventional and Modified Methods

      • Michihiro Yoshimura, Shota Nakamura, Hisao Ogawa
      Pages 189-198

    9. Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR

      • Ian M. Bird
      Pages 199-213

    10. New Advances in Microarrays Finding the Genes Causally Involved in Disease

      • Claudio Napoli
      Pages 215-233
Back to top

Keywords

About this book

A collection of new and essential molecular techniques for cardiovascular research. These readily reproducible methods range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using nonviral (polymers, liposomes, and antisense agents) and adenoviral vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. The book offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.

Reviews

From the reviews:

"This is an outstandingly interesting book..." - Biomedical Chromatography

"This book of 29 chapters by 73 contributors is exclusively for researchers in hypertension." (American Journal of Cardiology, June, 2007)

Editors and Affiliations

  • Moyne Institute for Preventive Medicine, Trinity College, Dublin, Ireland

    Jérôme P. Fennell

  • Division of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK

    Andrew H. Baker

Bibliographic Information

  • Book Title: Hypertension

  • Book Subtitle: Methods and Protocols

  • Editors: Jérôme P. Fennell, Andrew H. Baker

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598501

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-323-7Published: 27 December 2004

  • Softcover ISBN: 978-1-61737-493-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-850-2Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVI, 502

  • Number of Illustrations: 98 b/w illustrations

  • Topics: Cardiology

Publish with us

Policies and ethics

Back to top

Search

Navigation