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DNA Viruses

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Paul M. Lieberman

    1. The Wistar Institute, Philadelphia

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Part of the book series: Methods in Molecular Biology (MIMB, volume 292)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Viral Detection

    1. Front Matter

      Pages 1-81
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    2. Viral Detection

      • Feng Wang-Johanning, Gary L. Johanning
      Pages 3-13

    3. Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1

      • Servi J. C. Stevens, Sandra A. W. M. Verkuijlen, Jaap M. Middeldorp
      Pages 15-25

    4. Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR

      • Servi J. C. Stevens, Antoinette A. T. P. Brink, Jaap M. Middeldorp
      Pages 27-37

    5. Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo

      • Donna R. Hochberg, David A. Thorley-Lawson
      Pages 39-56

    6. Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo

      • Nancy M. Sawtell
      Pages 57-71

    7. Reporter Cell Lines for the Detection of Herpes Simplex Viruses

      • Szu-Hao Kung
      Pages 73-81

  3. Virus Structure And Imaging

    1. Front Matter

      Pages 83-171
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    2. Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy

      • Alexander J. Malkin, Marco Plomp, Alexander McPherson
      Pages 85-108

    3. Studying the Structure of Large Viruses With Multiresolution Imaging

      • Carmen San
      Pages 109-128

    4. Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence

      • Helle Lone, Bodil Norrild
      Pages 129-142

    5. Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy

      • Helle Lone, Bodil Norrild
      Pages 143-160

    6. FTIR Microscopy Detection of Cells Infected With Viruses

      • Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, Mahmoud Huleihel
      Pages 161-172

  4. Virus Entry

    1. Front Matter

      Pages 173-211
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    2. The JC Virus-Like Particle Overlay Assay

      • Hirofumi Sawa, Rika Komagome
      Pages 175-185

    3. Analysis of Fusion Using a Virus-Free Cell Fusion Assay

      • Marisa P. McShane, Richard Longnecker
      Pages 187-195

    4. Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells

      • Martin Sapp, Hans-Christoph Selinka
      Pages 197-211

  5. Gene Expression

    1. Front Matter

      Pages 213-244
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    2. Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence

      • Brian A. Van Tine, Thomas R. Broker, Louise T. Chow
      Pages 215-230
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Keywords

About this book

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Reviews

"Good presentation...laboratories working with DNA viruses will find ample useful material...." - Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • The Wistar Institute, Philadelphia

    Paul M. Lieberman

Bibliographic Information

  • Book Title: DNA Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Paul M. Lieberman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259848X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-353-4Published: 22 October 2004

  • Softcover ISBN: 978-1-62703-810-2Published: 22 November 2014

  • eBook ISBN: 978-1-59259-848-9Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 498

  • Topics: Infectious Diseases, Virology, Biochemistry, general

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