Cartilage and Osteoarthritis

1. Front Matter

   Pages i-xiii

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2. Generation and Use of Transgenic Mice As Models of Osteoarthritis

   • Anna-Marja Säämänen, Eero Vuorio

   Pages 1-23

3. Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide

   • Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, Patrick Garnero

   Pages 25-37

4. Histological and Immunohistological Studies on Cartilage

   • James Melrose, Susan Smith, Peter Ghosh

   Pages 39-63

5. Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe

   • James Melrose, Susan Smith

   Pages 65-78

6. Methods for Cartilage and Subchondral Bone Histomorphometry

   • Philippe Pastoureau, Agnès Chomel

   Pages 79-91

7. Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritis

   • Martin Judex, Elena Neumann, Steffan Gay, Ulf Müller-Ladner

   Pages 93-105

8. Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopy

   • Stephan Soeder, Alexander Kuhlmann, Thomas Aigner

   Pages 107-125

9. Molecular and Biochemical Assays of Cartilage Components

   • Caroline D. Hoemann

   Pages 127-156

10. Mechanical Characterization of Native and Tissue-Engineered Cartilage

    • Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz et al.

    Pages 157-190

11. Noninvasive Study of Human Cartilage Structure by MRI

    • Felix Eckstein

    Pages 191-217

12. High-Resolution MRI Techniques for Studies in Small-Animal Models

    • Olivier Beuf

    Pages 219-229

13. High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography

    • Lydia Wachsmuth, Klaus Engelke

    Pages 231-248

14. High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes

    • Amena Saïed, Pascal Laugier

    Pages 249-265

15. Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance

    • Jürgen Schiller, Daniel Huster, Beate Fuchs, Lama Naji, Jörn Kaufmann, Klaus Arnold

    Pages 267-285

16. Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage

    • Jürgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, Klaus Arnold

    Pages 287-302

17. Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods

    • Daniel Huster, Jürgen Schiller, Klaus Arnold

    Pages 303-318

18. Computerized Protocol for Anatomical and Functional Studies of Joints

    • Sandra Martelli, Stefano Zaffagnini

    Pages 319-338

19. Back Matter

    Pages 339-343

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An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

• Arthritis
• bone
• cartilage
• computed tomography
• computed tomography (CT)
• imaging
• joint
• metabolism
• osteoarthritis
• pathophysiology
• physiology
• protein
• research
• sonography
• tissue

"This is a high quality laboratory manual that provides detailed instructions for conducting experiments directed at studying the role of cartilage in osteoarthritis. Overall, this would be a worthwhile book to keep in the laboratory for anyone studying the molecular mechanisms of connective tissue diseases."-Doody's Health Sciences Book Review Journal

• Division de Rhumatologie, Institut de Recherches Servier, Suresnes, France

  Frédéric Ceuninck, Massimo Sabatini, Philippe Pastoureau

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