Signal Transduction Protocols

1. Front Matter

   Pages i-xii

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2. Making Protein Immunoprecipitates

   • Elaine A. Elion, Yunmei Wang

   Pages 1-14

3. Signal Transduction Inhibitors in Cellular Function

   • Maofu Fu, Chenguang Wang, Xueping Zhang, Richard G. Pestell

   Pages 15-36

4. Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets

   • Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, Natalie G. Ahn

   Pages 37-49

5. A High-Throughput Mammalian Cell-Based Transient Transfection Assay

   • Daniel J. Noonan, Kenneth Henry, Michelle L. Twaroski

   Pages 51-65

6. Determining Protein Half-Lives

   • Pengbo Zhou

   Pages 67-77

7. Assaying Protein Kinase Activity

   • Jan Brábek, Steven K. Hanks

   Pages 79-90

8. Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach

   • Francesca Zappacosta, Michael J. Huddleston, Roland S. Annan

   Pages 91-110

9. Studies of Calmodulin-Dependent Regulation

   • Paul C. Brandt, Thomas C. Vanaman

   Pages 111-127

10. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation

    • Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, Emery H. Bresnick

    Pages 129-146

11. Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation

    • Laurent Kuras

    Pages 147-162

12. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins

    • Douglas A. Andres

    Pages 163-173

13. Nucleocytoplasmic Glycosylation, O-GlcNAc

    • Natasha Elizabeth Zachara, Win Den Cheung, Gerald Warren Hart

    Pages 175-194

14. Techniques in Protein Methylation

    • Jaeho Lee, Donghang Cheng, Mark T. Bedford

    Pages 195-208

15. Assaying Lipid Phosphate Phosphatase Activities

    • Gil-Soo Han, George M. Carman

    Pages 209-216

16. Assaying Phosphoinositide Phosphatases

    • Gregory S. Taylor, Jack E. Dixon

    Pages 217-227

17. Assaying Phospholipase A2 Activity

    • Christina C. Leslie, Michael H. Gelb

    Pages 229-242

18. Measurement and Immunofluorescence of Cellular Phosphoinositides

    • Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, Daryll B. DeWald

    Pages 243-258

19. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer

    • Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, Manuela Zaccolo

    Pages 259-270

20. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET

    • Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, Albrecht G. von Arnim

    Pages 271-286

In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.

Reviews of the first edition:
...this well-organized volume is clearly among the most viable protocol books published in this field and will turn out to be a helpful tool for many laboratories.-FEBS Letters

• Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington

  Robert C. Dickson, Michael D. Mendenhall

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