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  • Book
  • © 2004

Bioconjugation Protocols

Strategies and Methods

Editors:

  1. Christof M. Niemeyer

    1. Fachbereich Chemie der Universität Dortmund, Dortmund, Germany

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Part of the book series: Methods in Molecular Biology (MIMB, volume 283)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Antibody and Enzyme Conjugates

    1. Front Matter

      Pages 1-1
      PDF

    2. Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies

      • Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, Vladimir R. Muzykantov
      Pages 3-19

    4. Smart Polymer-Streptavidin Conjugates

      • Patrick S. Stayton, Zhongli Ding, Allan S. Hoffman
      Pages 37-43

    5. Conjugates of Peptides and Proteins to Polyethylene Glycols

      • Margherita Morpurgo, Francesco M. Veronese
      Pages 45-70

    6. Chemical Production of Bispecific Antibodies

      • Robert F. Graziano, Paul Guptill
      Pages 71-85

  3. Nucleic Acid Conjugates

    1. Front Matter

      Pages 125-125
      PDF

    2. Fluorescent Sample Labeling for DNA Microarray Analyses

      • Verena Beier, Andrea Bauer, Michael Baum, Jörg D. Hoheisel
      Pages 127-135

    4. Sequence-Specific DNA Labeling Using Methyltransferases

      • Goran Pljevaljčić, Falk Schmidt, Alexander Peschlow, Elmar Weinhold
      Pages 145-161

    6. Covalent Coupling of DNA Oligonucleotides and Streptavidin

      • Florian Kukolka, Marina Lovrinovic, Ron Wacker, Christof M. Niemeyer
      Pages 181-196

    8. Synthesis of Peptide Nucleic Acid-Peptide Conjugates

      • Kunihiro Kaihatsu, David R. Corey
      Pages 207-214

  4. Glycosyl and Lipid Conjugates

    1. Front Matter

      Pages 215-215
      PDF

    2. Protein Lipidation

      • Jürgen Kuhlmann
      Pages 217-220

    3. Synthesis of Lipidated Peptides

      • Ines Heinemann, Martin Völkert, Herbert Waldmann
      Pages 221-232
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About this book

There are a number of outstanding volumes that provide a comprehensive overview of bioconjugation techniques. However, many of the conventional approaches to the synthesis of chemically modified protein conjugates lack efficient means to control the stoichiometry of conjugation, as well as the s- cific site of attachment of the conjugated moiety. Moreover, the recent dev- opments in microarray technologies as well as in nanobiotechnology—a novel field of research rapidly evolving at the crossroads of physics, chemistry, b- technology, and materials science—call for a summary of modern bioconjugation strategies to overcome the limitations of the classical approaches. Bioconjugation Protocols: Methods and Strategies is intended to provide an update of many of the classic techniques and also to introduce and summarize newer approaches that go beyond the pure biomedical applications of bioconjugation. The purpose of Bioconjugation Protocols: Methods and Str- egies is therefore to provide instruction and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical diagnostics, to therapeutics, to biomaterials. Part I contains seven protocols for the preparation of protein conjugates.
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Reviews

"...provides highlights for scientists who are pioneering in the synthesis of biofunctional macromolecules, as well as providing resources to all levels of scientists who are engaged in the fields of diagnostics, biomaterials, and pharmacotherapeutics." - Journal of Medicinal Chemistry
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Editors and Affiliations

  • Fachbereich Chemie der Universität Dortmund, Dortmund, Germany

    Christof M. Niemeyer

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Bibliographic Information

  • Book Title: Bioconjugation Protocols

  • Book Subtitle: Strategies and Methods

  • Editors: Christof M. Niemeyer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598137

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • eBook ISBN: 978-1-59259-813-7Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 332

  • Topics: Cell Biology

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Buy it now

eBook USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

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