Nitric Oxide Protocols

1. Front Matter

   Pages i-xi

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2. Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO

   • Reinhard Berkels, Svenja Purol-Schnabel, Renate Roesen

   Pages 1-8

3. High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples

   • Dmitri Y. Boudko

   Pages 9-19

4. Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis

   • Edward Morcos, N. Peter Wiklund

   Pages 21-34

5. In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode

   • Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, Kotaro Oka

   Pages 35-43

6. Measurement of Exhaled Nitric Oxide

   • Peter A. Steerenberg, Jan G. C. van Amsterdam

   Pages 45-68

7. Nitric Oxide Imaging in Neurons Using Confocal Microscopy

   • Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, Mu Zhang

   Pages 69-80

8. Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry

   • Dimitrios Tsikas

   Pages 81-103

9. A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts

   • Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, Stanley Salmons

   Pages 105-111

10. Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization

    • Olivier Braissant

    Pages 113-124

11. Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase

    • Yi Chu, Frank M. Faraci

    Pages 125-132

12. Dioxygen-Dependent Metabolism of Nitric Oxide

    • Paul R. Gardner, Anne M. Gardner, Craig K. Hallstrom

    Pages 133-150

13. Proteomic Method for Identification of Tyrosine-Nitrated Proteins

    • Kulwant S. Aulak, Thomas Koeck, John W. Crabb, Dennis J. Stuehr

    Pages 151-165

14. Immunohistochemical Detection of S-Nitrosylated Proteins

    • Andrew J. Gow, Christiana W. Davis, David Munson, Harry Ischiropoulos

    Pages 167-172

15. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction

    • Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, Philip J. Kadowitz

    Pages 173-185

16. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats

    • Yoshitaka Hirooka, Koji Sakai

    Pages 187-200

17. Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase

    • Masahide Kuroki

    Pages 201-211

18. Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors

    • Yoshikazu Maeda, Kazuyuki Shimada, Uichi Ikeda

    Pages 213-224

19. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer

    • Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, Melina R. Kibbe

    Pages 225-234

20. Back Matter

    Pages 235-246

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Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.

From reviews of the first edition...

"...an excellent book...offers newcomers and experienced scientists a wealth of important practical information that will be of inestimable value both to newcomers and experienced research groups...an essential resource for every laboratory involved in NO-related work..." -Angewandte Chemie

"...a very useful handbook and definitely a must for researchers working at the bench in any field related one way or another to NO." -Psychoneuroendocrinology

"...This book is a must for laboratories with an interest in nitric oxide..." -British Journal of Anesthesia

"...will certainly find its way into many laboratories entering the exciting field of nitric oxide research. " -Quarterly Review of Biology

• Department of Physiology, College of Medicine, University of Tennessee, Memphis

  Aviv Hassid

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