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  • Book
  • © 2004

Autoimmunity

Methods and Protocols

Editors:

  1. Andras Perl

    1. Departments of Medicine, Microbiology, and Immunology, State University of New York, Upstate Medical University, College of Medicine, Syracuse

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 102)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Pathogenesis and Spectrum of Autoimmunity

  3. Models of Pathogenesis in Human Autoimmune Disorders

    1. Front Matter

      Pages 9-9
      PDF

    2. Mapping the Systemic Lupus Erythematosus Susceptibility Genes

      • Swapan K. Nath, Jennifer A. Kelly, John B. Harley, R. Hal Scofield
      Pages 11-29

    3. T-Cell Signaling Abnormalities in Human Systemic Lupus Erythematosus

      • Madhusoodana P. Nambiar, Sandeep Krishnan, George C. Tsokos
      Pages 31-47

    4. TCR ζ-Chain Abnormalities in Human Systemic Lupus Erythematosus

      • Madhusoodana P. Nambiar, Sandeep Krishnan, Vishal G. Warke, George C. Tsokos
      Pages 49-72

    5. Protein Kinase A and Signal Transduction in T Lymphocytes

      • Islam U. Khan, Gary M. Kammer
      Pages 73-85

    6. Apoptosis and Mitochondrial Dysfunction in Lymphocytes of Patients With Systemic Lupus Erythematosus

      • Andras Perl, Gyorgy Nagy, Peter Gergely, Ferenc Puskas, Yueming Qian, Katalin Banki
      Pages 87-114

    7. Methods for Inducing Apoptosis

      • Kathryn M. Roberts, Antony Rosen, Livia A. Casciola-Rosen
      Pages 115-128

    8. Measurement of Cytokines in Autoimmune Disease

      • Kyriakos A. Kirou, Christina Lee, Mary K. Crow
      Pages 129-154

  4. Animal Models of Autoimmunity

    1. Front Matter

      Pages 173-173
      PDF

    2. Animal Models for Autoimmune Myocarditis and Autoimmune Thyroiditis

      • Daniela Čiháková, Rajni B. Sharma, DeLisa Fairweather, Marina Afanasyeva, Noel R. Rose
      Pages 175-193

    3. Animal Models of Insulin-Dependent Diabetes

      • Edwin Liu, Liping Yu, Hiroaki Moriyama, George S. Eisenbarth
      Pages 195-212

    4. Generation, Maintenance, and Adoptive Transfer of Diabetogenic T-Cell Lines/Clones From the Nonobese Diabetic Mouse

      • Martha J. Milton, Michelle Poulin, Clayton Mathews, Jon D. Piganelli
      Pages 213-225

    5. Experimental Use of Murine Lupus Models

      • Stanford L. Peng
      Pages 227-272

    7. Murine Models of Lupus Induced by Hypomethylated T Cells

      • Bruce Richardson, Donna Ray, Raymond Yung
      Pages 285-294

    8. The Mouse Model of Collagen-Induced Arthritis

      • David D. Brand, Andrew H. Kang, Edward F. Rosloniec
      Pages 295-312

    9. Proteoglycan Aggrecan-Induced Arthritis

      • Tibor T. Glant, Katalin Mikecz
      Pages 313-338
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About this book

“Research is to see what everybody else has seen, and to think what nobody else has thought. ” — Albert Szentgyörgyi Autoimmunity: Methods and Protocols is intended to serve as a ready-to-use guide to establish and interrogate human and animal models of autoimmune diseases. The first chapter, “Pathogenesis and Spectrum of Autoimmunity,” discusses major hypotheses driving this most tantalizing area of research since Paul Ehrlich proposed the concept of autoimmunity in 1900. Considering the great diversity and ever-changing spectrum of autoimmunity, it has not been possible to include models and experimental protocols for each known disorder. Rather, several chapters have been devoted to the most prevalent and complex diseases, such as rheumatoid arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, and multiple sclerosis. The chapters are contributed by laboratories actively using the models presented. Each chapter contains an introductory section that discusses the relevance of the model for a particular disease and for autoimmunity in general. Part I contains methods and protocols to assess immunological and biochemical pathways relevant for disease pathogenesis. Chapters in this section focus on methods to identify susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor and signal processing via protein kinases, identification and enumeration of autoantigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Part II contains protocols to establish and assess inflammatory arthritis, systemic lupus erythematosus, myocarditis, thyroiditis, experimental autoimmune encepha- myelitis, insulin-dependent diabetesmellitus, scleroderma, uveitis, and vitiligo.
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Editors and Affiliations

  • Departments of Medicine, Microbiology, and Immunology, State University of New York, Upstate Medical University, College of Medicine, Syracuse

    Andras Perl

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Bibliographic Information

  • Book Title: Autoimmunity

  • Book Subtitle: Methods and Protocols

  • Editors: Andras Perl

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598056

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • eBook ISBN: 978-1-59259-805-2Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 434

  • Topics: Immunology

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  • Own it forever

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