Skip to main content
SpringerLink
Log in

Photosynthesis Research Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Robert Carpentier

    1. Départment de Chimie-Biologie (GREIB), Université du Québec à Trois-Rivières, Trois-Rivières, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 274)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids

    • Juan Cuello, MarÍa José Quiles
    Pages 1-9

  3. Isolation of Photosystem I Particles From Spinach

    • Tetsuo Hiyama
    Pages 11-17

  4. Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii

    • Velupillai M. Ramesh, Andrew N. Webber
    Pages 19-28

  5. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants

    • Yasusi Yamamoto, Shinsuke Sakuma, Jian-Ren Shen
    Pages 29-36

  6. Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803

    • Dmitrii V. Vavilin
    Pages 37-47

  7. Isolation of Photosystem I Reaction Center Preparation From Spinach

    • Tetsuo Hiyama
    Pages 49-51

  8. Isolation of Photosystem II Reaction Center Complexes From Plants

    • Michael Seibert, Inmaculada Yruela, Rafael Picorel
    Pages 53-62

  9. Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach

    • Tetsuo Hiyama
    Pages 63-66

  10. Purification and Crystallization of the Cytochrome b 6 f Complex in Oxygenic Photosynthesis

    • Huamin Zhang, William A. Cramer
    Pages 67-78

  11. Purification of Plastocyanin and Cytochrome c 6 From Plants, Green Algae, and Cyanobacteria

    • José A. Navarro, Manuel Hervás, Miguel A. De la Rosa
    Pages 79-92

  12. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex

    • Wolfgang Rühle, Harald Paulsen
    Pages 93-103

  13. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions

    • Ilian Simidjiev, Zsuzsanna Várkonyi, Gyözö Garab
    Pages 105-114

  14. Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

    • Grzegorz Jackowski
    Pages 115-128

  15. Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants

    • Rafael Picorel, Miguel Alfonso, Michael Seibert
    Pages 129-135

  16. The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry

    • Tessa Pocock, Marianna Król, Norman P. A. Huner
    Pages 137-148

  17. Isolation and Identification of Chloroplast Lipids

    • Norihiro Sato, Mikio Tsuzuki
    Pages 149-157

  18. DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents

    • Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, Stavroula Diamantoglou
    Pages 159-171

  19. Incorporation and Analysis of LHCII in Model Systems

    • Wieslaw I. Gruszecki
    Pages 173-181

  20. Photosystem II Reconstitution Into Proteoliposomes

    • Mário Fragata
    Pages 183-204
Back to top

About this book

Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynthesis research. Photosynthesis Research Protocols contains a broad range of general and fundamental me- ods that are commonly used by plant biochemists, physiologists, and mole- lar biologists. This book is thus intended as a source of information for scientists working on any of the multiple aspects of photosynthesis, and should be of great interest to a multidisciplinary field of research involving agric- ture, biochemistry, biotechnology, botany, cell biology, environmental s- ences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc.

Reviews

From the reviews:

"This book series is well known as a competent tool for research protocols in all fields of life science. … The book addresses either beginners in photosynthesis research or experienced researchers who want to extend their methodological experience. … I can recommend the book as basic literature for advanced courses in photosynthesis. … In conclusion, this is an excellent book which can be recommended for all laboratories involved in photosynthesis education and research." (Christian Wilhelm, Journal of Plant Physiology, Vol. 162 (6), 2005)

Editors and Affiliations

  • Départment de Chimie-Biologie (GREIB), Université du Québec à Trois-Rivières, Trois-Rivières, Canada

    Robert Carpentier

Bibliographic Information

  • Book Title: Photosynthesis Research Protocols

  • Editors: Robert Carpentier

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597998

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • eBook ISBN: 978-1-59259-799-4Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 346

  • Topics: Plant Sciences

Publish with us

Policies and ethics

Back to top

Search

Navigation