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Capillary Electrophoresis of Proteins and Peptides

  • Book
  • © 2004

Overview

Editors:
  1. Mark A. Strege

    1. Eli Lilly and Company, Indianapolis

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    You can also search for this editor in PubMed   Google Scholar

  2. Avinash L. Lagu

    1. Lilly Research Laboratories, Indianapolis

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 276)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis

    • Charles A. Lucy, Nicole E. Baryla, Ken K.-C. Yeung
    Pages 1-14

  3. Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillary Electrophoresis With Laser-Induced Fluorescence Detection

    • Hossein Ahmadzadeh, Norman J. Dovichi, Sergey Krylov
    Pages 15-27

  4. On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection

    • Hossein Ahmadzadeh, Sergey Krylov
    Pages 29-38

  5. Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications

    • John Sowell, Jozef Salon, Lucjan Strekowski, Gabor Patonay
    Pages 39-75

  6. Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes

    • Vadim Klyushnichenko
    Pages 77-120

  7. Capillary Electrophoresis of Proteins in a Quality Control Environment

    • David L. Good, Stacey Cummins-Bitz, Raeann M. Fields, Brian K. Nunnally
    Pages 121-136

  8. Analysis of Neutral N-Linked Oligosaccharides From Antibodies Using Free-Solution Capillary Electrophoresis in Bare Fused-Silica Capillaries

    • Jeffrey S. Patrick, Brenda P. Rener, Gregory S. Clanton, Avinash L. Lagu
    Pages 137-151

  9. Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions

    • Maryam Azad, John Kaddis, Valerie Villareal, Lili Hernandez, Catherine Silverio, Frank A. Gomez
    Pages 153-168

  10. Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis

    • Hee Seung Kim, John Austin, David S. Hage
    Pages 169-187

  11. Using Charge Ladders and Capillary Electrophoresis to Measure the Charge, Size, and Electrostatic Interactions of Proteins

    • Upma Sharma, Jeffrey D. Carbeck
    Pages 189-216

  12. Frontal Analysis Continuous Capillary Electrophoresis for Protein-Polyelectrolyte Binding Studies

    • Emek Seyrek, Toshiaki Hattori, Paul L. Dubin
    Pages 217-228

  13. Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection

    • Jiaqi Wu, Xing-Zheng Wu, Tiemin Huang, Janusz Pawliszyn
    Pages 229-252

  14. Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins

    • Mehdi Moini
    Pages 253-290

  15. Capillary Isoelectric Focusing-Mass Spectrometry of Proteins and Protein Complexes

    • Suzana Martinović, Ljiljana PaÅ¡a-Tolić, Richard D. Smith
    Pages 291-304

  16. Integrated System for Rapid Proteomics Analyses Using Microfluidic Devices Coupled to Nanoelectrospray Mass Spectrometry

    • Jianjun Li, Tammy-Lynn Tremblay, Jed Harrison, Pierre Thibault
    Pages 305-324

  17. Back Matter

    Pages 325-332
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About this book

Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Editors and Affiliations

  • Eli Lilly and Company, Indianapolis

    Mark A. Strege

  • Lilly Research Laboratories, Indianapolis

    Avinash L. Lagu

Bibliographic Information

  • Book Title: Capillary Electrophoresis of Proteins and Peptides

  • Editors: Mark A. Strege, Avinash L. Lagu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259798X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-017-5Published: 03 June 2004

  • Softcover ISBN: 978-1-61737-307-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-798-7Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 332

  • Topics: Biochemistry, general

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