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  • Book
  • © 2004

Platelets and Megakaryocytes

Volume 1: Functional Assays

Editors:

  1. Jonathan M. Gibbins

    1. School of Animal and Microbial Sciences, The University of Reading, Reading, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Martyn P. Mahaut-Smith

    1. Department of Physiology, University of Cambridge, Cambridge, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 272)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xix
    PDF

  2. Platelet Functional Assays

    1. Front Matter

      Pages 1-1
      PDF

    3. Preparation of Washed Platelet Suspensions From Human and Rodent Blood

      • Jean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckly, Béatrice Hechler, Christian Gachet
      Pages 13-28

    4. Platelet Counting

      • Paul Harrison, Carol Briggs, Samuel J. Machin
      Pages 29-46

    6. Platelet Aggregation

      • Gavin E. Jarvis
      Pages 65-76

    7. Platelet Aggregation in Whole Blood

      • Gavin E. Jarvis
      Pages 77-87

    8. Secretion From Dense Granules

      • M. Fred Heath
      Pages 89-93

    9. Platelet Dense-Granule Secretion

      • David Crosby, Alastair W. Poole
      Pages 95-96

    10. Analysis of the Releasable Nucleotides of Platelets

      • Joachim Jankowski, Lars Henning, Hartmut Schlüter
      Pages 97-108

    11. Studies of Secretion Using Permeabilized Platelets

      • Tara W. Rutledge, Sidney W. Whiteheart
      Pages 109-120

    12. Measurement of Platelet Arachidonic Acid Metabolism

      • Richard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, Rosemary J. Keogh
      Pages 121-133

    13. Measurement of the Platelet Procoagulant Response

      • Johan W. M. Heemskerk, Paul Comfurius, Marion A. H. Feijge, Edouard M. Bevers
      Pages 135-144

    15. Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns

      • Renata Polanowska-Grabowska, Adrian R. L. Gear
      Pages 153-163

    16. Techniques to Examine Platelet Adhesive Interactions Under Flow

      • Suhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, Shaun P. Jackson
      Pages 165-186

    17. In Vivo Models of Platelet Function and Thrombosis

      • Shahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Celi et al.
      Pages 187-197
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About this book

12 The average human body has in the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.
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Reviews

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology
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Editors and Affiliations

  • School of Animal and Microbial Sciences, The University of Reading, Reading, UK

    Jonathan M. Gibbins

  • Department of Physiology, University of Cambridge, Cambridge, UK

    Martyn P. Mahaut-Smith

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Bibliographic Information

  • Book Title: Platelets and Megakaryocytes

  • Book Subtitle: Volume 1: Functional Assays

  • Editors: Jonathan M. Gibbins, Martyn P. Mahaut-Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597823

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-101-1Published: 01 July 2004

  • Softcover ISBN: 978-1-61737-356-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-782-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 385

  • Topics: Hematology

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Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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