Pancreatic Cancer

1. Front Matter

   Pages i-xi

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2. Identification and Analysis of Precursors to Invasive Pancreatic Cancer

   • Ralph H. Hruban, Robb E. Wilentz, Anirban Maitra

   Pages 1-13

3. Optimal Molecular Profiling of Tissue and Tissue Components

   • G. Steven Bova, Isam A. Eltoum, John A. Kiernan, Gene P. Siegal, Andra R. Frost, Carolyn J. M. Best et al.

   Pages 15-66

4. Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia

   • Robb E. Wilentz, Ayman Rahman, Pedram Argani, Christine Iacobuzio-Donahue

   Pages 67-88

5. Practical Methods for Tissue Microarray Construction

   • Helen L. Fedor, Angelo M. De Marzo

   Pages 89-101

6. Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis

   • Kimberly Walter, James Eshleman, Michael Goggins

   Pages 103-111

7. Culture and Immortalization of Pancreatic Ductal Epithelial Cells

   • Terence Lawson, Michel Ouellette, Carol Kolar, Michael Hollingsworth

   Pages 113-122

8. DNA Methylation Analysis in Human Cancer

   • Carmelle D. Curtis, Michael Goggins

   Pages 123-136

9. Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance

   • Hsueh-Wei Chang, Ie-Ming Shih

   Pages 137-141

10. Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes

    • Antoinette Hollestelle, Mieke Schutte

    Pages 143-159

11. Serial Analyses of Gene Expression (SAGE)

    • Jia Le Dai

    Pages 161-174

12. Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma

    • David E. Misek, Rork Kuick, Samir M. Hanash, Craig D. Logsdon

    Pages 175-187

13. Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach

    • Christophe Rosty, Michael Goggins

    Pages 189-197

14. Detection of Telomerase Activity in Patients with Pancreatic Cancer

    • Kazuhiro Mizumoto, Masao Tanaka

    Pages 199-205

15. Serological Analysis of Expression cDNA Libraries (SEREX)

    • Yao-Tseng Chen, Ali O. Gure, Matthew J. Scanlan

    Pages 207-216

16. Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses

    • Paul J. Grippo, Eric P. Sandgren

    Pages 217-243

17. Strategies for the Use of Site-Specific Recombinases in Genome Engineering

    • Julie R. Jones, Kathy D. Shelton, Mark A. Magnuson

    Pages 245-257

18. Primary Explant Cultures of Adult and Embryonic Pancreas

    • Farzad Esni, Yoshiharu Miyamoto, Steven D. Leach, Bidyut Ghosh

    Pages 259-271

19. Zebrafish as a Model for Pancreatic Cancer Research

    • Nelson S. Yee, Michael Pack

    Pages 273-298

20. Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

    • Dan Laheru, Barbara Biedrzycki, Amy M. Thomas, Elizabeth M. Jaffee

    Pages 299-327

Pancreatic ductal adenocarcinoma is the fourth leading cause of cancer death in the United States. Annually approximately 30,000 Americans are diagnosed with the disease and most will die from it within five years. P- creatic ductal adenocarcinoma is unique because of its late onset in age, high mortality, small tumor samples infiltrated with normal cells, and a lack of both early detection and effective therapies. Some of these characteristics have made studying this disease a challenge. Pancreatic cancer develops as a result of the accumulation of genetic alterations in cancer-causing genes, such as the oncogenes and the tumor-s- pressor genes. In the last decade, major progress has been made in identifying important oncogenes and tumor-suppressor genes for the disease. In Panc- atic Cancer: Methods and Protocols, we review the classical techniques that have contributed to the advances in pancreatic research and introduce new strategies that we hope will add to future breakthroughs in the field of cancer biology. Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of s- radic and familial pancreatic cancer. It covers topics from in vitro cell c- tures to in vivo mouse models, DNA to protein manipulation, and mutation analyses to treatment development. We believe that our book will prove an invaluable source of proven protocols for those who are interested in either basic or translational research in pancreatic cancer.

• DNA
• antigen
• cancer
• carcinoma
• cell
• cell culture
• gene
• gene expression
• pancreas
• pancreatic cancer
• screening
• tumor
• vaccine

"...a worthwhile contribution from a team of experts." Doody's Health Sciences and Book Review Journal

"...a welcome addition to the field...an excellent collection of protocols for a broad range of advanced techniques relevant to many aspects of pancreatic cancer research." -Karger

• Departments of Otolaryngology/Head and Neck Surgery and Pathology, Columbia University, New York

  Gloria H. Su

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