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  • Book
  • © 2004

Recombinant Gene Expression

Reviews and Protocols

Editors:

  1. Paulina Balbás

    1. Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Argelia Lorence

    1. Virginia Polytechnic Institute and State University, Blacksburg

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 267)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. General Issues About Recombinant Gene Expression

    1. Front Matter

      Pages 1-1
      PDF

    3. Production of Recombinant Proteins

      • Laura A. Palomares, Sandino Estrada-Moncada, Octavio T. Ramírez
      Pages 15-51

  3. Prokaryotes

    1. Front Matter

      Pages 75-75
      PDF

    2. Back to Basics

      • Paulina Balbás, Francisco Bolívar
      Pages 77-90

    4. Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli

      • Jean-Claude Boulain, Frédéric Ducancel
      Pages 101-112

    5. Overexpression of Chromosomal Genes in Escherichia coli

      • Fernando Valle, Noemí Flores
      Pages 113-122

    7. Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli

      • Sylvie Le Borgne, Francisco Bolívar, Guillermo Gosset
      Pages 135-143

    8. Copy-Control pBAC/oriV Vectors for Genomic Cloning

      • Jadwiga Wild, Waclaw Szybalski
      Pages 145-154

    9. Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV

      • Jadwiga Wild, Waclaw Szybalski
      Pages 155-167

    10. Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation

      • James R. Swartz, Michael C. Jewett, Kim A. Woodrow
      Pages 169-182

    12. Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria

      • Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto, Constantin Drainas
      Pages 209-223

    13. Recombinant Protein Production in Antarctic Gram-Negative Bacteria

      • Angela Duilio, Maria Luisa Tutino, Gennaro Marino
      Pages 225-237

  4. Fungi

    1. Front Matter

      Pages 239-239
      PDF

    2. Recombinant Protein Production in Yeasts

      • Danilo Porro, Diethard Mattanovich
      Pages 241-258
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About this book

Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
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Reviews

From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology

"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today
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Editors and Affiliations

  • Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México

    Paulina Balbás

  • Virginia Polytechnic Institute and State University, Blacksburg

    Argelia Lorence

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Bibliographic Information

  • Book Title: Recombinant Gene Expression

  • Book Subtitle: Reviews and Protocols

  • Editors: Paulina Balbás, Argelia Lorence

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597742

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-262-9Published: 20 July 2004

  • Softcover ISBN: 978-1-61737-460-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-774-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 508

  • Topics: Cell Biology

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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