Overview
- Editors:
-
-
Paulina Balbás
-
Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México
-
Argelia Lorence
-
Virginia Polytechnic Institute and State University, Blacksburg
Access this book
Other ways to access
Table of contents (33 protocols)
-
-
General Issues About Recombinant Gene Expression
-
-
-
- Laura A. Palomares, Sandino Estrada-Moncada, Octavio T. Ramírez
Pages 15-51
-
-
Prokaryotes
-
-
- Paulina Balbás, Francisco Bolívar
Pages 77-90
-
- Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, Glenn L. Wilson
Pages 91-100
-
- Jean-Claude Boulain, Frédéric Ducancel
Pages 101-112
-
- Fernando Valle, Noemí Flores
Pages 113-122
-
- Lu Zhou, Ke Zhang, Barry L. Wanner
Pages 123-134
-
- Sylvie Le Borgne, Francisco Bolívar, Guillermo Gosset
Pages 135-143
-
- Jadwiga Wild, Waclaw Szybalski
Pages 145-154
-
- Jadwiga Wild, Waclaw Szybalski
Pages 155-167
-
- James R. Swartz, Michael C. Jewett, Kim A. Woodrow
Pages 169-182
-
- Carmen Vargas, Joaquín J. Nieto
Pages 183-208
-
- Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto, Constantin Drainas
Pages 209-223
-
- Angela Duilio, Maria Luisa Tutino, Gennaro Marino
Pages 225-237
-
Fungi
-
Front Matter
Pages 239-239
-
- Danilo Porro, Diethard Mattanovich
Pages 241-258
About this book
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Reviews
From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology
"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today
Editors and Affiliations
-
Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México
Paulina Balbás
-
Virginia Polytechnic Institute and State University, Blacksburg
Argelia Lorence
