Overview
- Editors:
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Shaying Zhao
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The Institute for Genomic Research, Rockville
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Marvin Stodolsky
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Office of Biological Environmental Research, US Department of Energy, Germantown
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Table of contents (20 protocols)
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Front Matter
Pages i-xviii
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- Michael M. Weil, Rashmi Pershad, Ruoping Wang, Sheng Zhao
Pages 1-6
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- Scott E. Wenderfer, John J. Monaco
Pages 7-20
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- Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Daniel Pinkel, Donna G. Albertson
Pages 39-56
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- Zunde Wang, Angelika Longacre, Peter Engler
Pages 69-76
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- Nathan Mise, Philip Avner
Pages 77-87
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- Srividya Swaminathan, Shyam K. Sharan
Pages 89-106
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- Joep P. P. Muyrers, Youming Zhang, Vladmir Benes, Giuseppe Testa, Jeanette M. J. Rientjes, A. Francis Stewart
Pages 107-121
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- Giuseppe Testa, Kristina Vintersten, Youming Zhang, Vladmir Benes, Joep P. P. Muyrers, A. Francis Stewart
Pages 123-139
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- Kristina Vintersten, Giuseppe Testa, A. Francis Stewart
Pages 141-158
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- Vikki M. Marshall, Janette Allison, Tanya Templeton, Simon J. Foote
Pages 159-182
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- Deborah A. Swing, Shyam K. Sharan
Pages 183-198
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- Eva-Maria Borst, Irena Crnkovic-Mertens, Martin Messerle
Pages 221-239
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- Thomas G. H. Heister, Andrea Vögtlin, Lars Müller, Irma Heid, Cornel Fraefel
Pages 241-256
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- Markus Wagner, Ulrich H. Koszinowski
Pages 257-268
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- Eva-Maria Borst, György Pósfai, Frank Pogoda, Martin Messerle
Pages 269-279
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- Alistair McGregor, Mark R. Schleiss
Pages 281-302
About this book
Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects.
Editors and Affiliations
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The Institute for Genomic Research, Rockville
Shaying Zhao
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Office of Biological Environmental Research, US Department of Energy, Germantown
Marvin Stodolsky
