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Gene Expression Profiling

  • Book
  • © 2004

Overview

Editors:
  1. Richard A. Shimkets

    1. CuraGen Corporation, New Haven

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Part of the book series: Methods in Molecular Biology (MIMB, volume 258)

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Table of contents (10 protocols)

  1. Front Matter

    Pages i-x
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  2. Technical Considerations in Quantitating Gene Expression

    • Richard A. Shimkets
    Pages 1-6

  3. Gene Expression Quantitation Technology Summary

    • Richard A. Shimkets
    Pages 7-12

  4. Standardized RT-PCR and the Standardized Expression Measurement Center

    • James C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner, Cheryl A. Motten, Elizabeth A. Herness et al.
    Pages 13-41

  5. GeneCalling

    • Richard A. Shimkets
    Pages 43-51

  6. Invader Assay for RNA Quantitation

    • Marilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis, Sarah M. Olson, Robert W. Kwiatkowski
    Pages 53-69

  7. Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays

    • Jennifer Lescallett, Marina E. Chicurel, Robert Lipshutz, Dennise D. Dalma-Weiszhausz
    Pages 71-94

  8. Amplified Differential Gene Expression Microarray

    • Zhijian J. Chen, Kenneth D. Tew
    Pages 95-106

  9. Suppression Subtractive Hybridization

    • Denis V. Rebrikov, Sejal M. Desai, Paul D. Siebert, Sergey A. Lukyanov
    Pages 107-134

  10. Small Amplified RNA-SAGE

    • Catheline Vilain, Gilbert Vassart
    Pages 135-152

  11. Gene Expression Informatics

    • Martin Leach
    Pages 153-165

  12. Back Matter

    Pages 167-168
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About this book

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

Editors and Affiliations

  • CuraGen Corporation, New Haven

    Richard A. Shimkets

Bibliographic Information

  • Book Title: Gene Expression Profiling

  • Editors: Richard A. Shimkets

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597513

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-220-9Published: 24 February 2004

  • Softcover ISBN: 978-1-61737-429-6Published: 09 December 2010

  • eBook ISBN: 978-1-59259-751-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 168

  • Topics: Cell Biology

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