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Nitric Oxide Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Michael A. Titheradge

    1. University of Sussex, Brighton, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 100)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Enzymology of Nitric Oxide Synthases

    • Benjamin Hemmens, Bernd Mayer
    Pages 1-32

  3. Purification of the Inducible Nitric Oxide Synthase

    • Edward P. Garvey
    Pages 33-36

  4. Purification of the Constitutive Nitric Oxide Synthase

    • Edward P. Garvey
    Pages 37-42

  5. Cloning and Expression of Human Inducible Nitric Oxide Synthase

    • Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar
    Pages 43-49

  6. Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System

    • Ian G Charles, Neale Foxwell, Ann Chubb
    Pages 51-60

  7. Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO

    • Mark Salter, Richard G. Knowles
    Pages 61-65

  8. Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation

    • Richard G. Knowles, Mark Salter
    Pages 67-73

  9. Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation

    • James M. Cunningham, Richard C. Rayne
    Pages 75-82

  10. The Enzymatic Measurement of Nitrate and Nitrite

    • Michael A. Titheradge
    Pages 83-91

  11. Determination of NOS Activity Using Cyclic-GMP Formation

    • Suzanne G. Laychock
    Pages 93-100

  12. Determination of NO with a Clark-Type Electrode

    • Kurt Schmidt, Bernd Mayer
    Pages 101-109

  13. The Measurement of NO in Biological Systems Using Chemiluminescence

    • Evangelos D. Michelakis, Stephen L. Archer
    Pages 111-127

  14. Measurement of NO Using Electron Paramagnetic Resonance

    • S. Tsuyoshi Ohnishi
    Pages 129-153

  15. Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction

    • Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt
    Pages 155-161

  16. Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay

    • Paloma Martín-Sanz, Lisardo Boscá
    Pages 163-170

  17. Detection of NOS Isoforms by Western-Blot Analysis

    • Fiona S. Smith, Michael A. Titheradge
    Pages 171-180

  18. Immunohistochemical Localization of NOS Isoforms

    • Victoria Cattell, Karen Mosley
    Pages 181-190

  19. A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization

    • G. Michael Taylor, H. Terence Cook
    Pages 191-204

  20. Use of NO Donors in Biological Systems

    • Jayne M. Tullett, Daryl D. Rees
    Pages 205-214
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Reviews

"... the protocols are concise and easy to follow. They are, in fact, extraordinary in their detail...Nitric Oxide Protocols is a useful volume, and one that will certainly find its way into many laboratories entering the exciting field of nitric oxide research."-The Quarterly Review of Biology

Editors and Affiliations

  • University of Sussex, Brighton, UK

    Michael A. Titheradge

Bibliographic Information

  • Book Title: Nitric Oxide Protocols

  • Editors: Michael A. Titheradge

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597491

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-749-9Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 324

  • Number of Illustrations: 40 b/w illustrations

  • Topics: Biochemistry, general

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