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Table of contents (20 protocols)
About this book
Reviews
"...the editors have managed to successfully compile a brief review of cytotoxic drug resistance mechanisms and an extensive manual of protocols for laboratory experimentation in this extremely important area of research. It is clear that any laboratory scientist in this field must be aware of the difficulty of translation to the clinic of anti-drug resistance lead compounds, and should keep a copy of this book within reach. Congratulations to the editors for assembling an important mix of laboratory and clinical scientists to create this volume."-The Quarterly Review of Biology
"This book provides a comprehensive guide to a range of methods to a diverse range of drug resistance mechanisms including: Multidrug resistance (P-glycoprotein and MDR-1 gene analysis); cell sensitivity (clonogenic and MTT assays); Apoptosis (TUNEL, p 53 and Bcl-2); Glutathione-associated resistance (enzyme based glutathione assay and a HPLC method for reduced glutathione); DNA repair defects and damage (topoisomerase activity assays and DNA adduct measurements); 5-Fluorouracil metabolizing enzymes; Genetic analysis including microsatellite instability and fluorescence in situ hybridization....The methods are clearly described with each chapter consisting of an introduction and well described materials and methods sections. An extremely useful addition to most chapters was the "Notes" section where the respective authors suggest very useful hints, problem solving approaches and advice on the interpretation of results....the book is well written and useful addition to any laboratory investigating drug resistance in either clinical samples or tumor cell lines."-Cancer Forum
"...a useful collection of protocols that can be used in the oncology laboratory for studies on drug resistance and molecular pharmacology....It should find its place on the library shelves of many laboratories of oncology."- Annals of Oncology
Editors and Affiliations
Bibliographic Information
Book Title: Cytotoxic Drug Resistance Mechanisms
Editors: Robert Brown, Uta Böger-Brown
Series Title: Methods in Molecular Medicine
DOI: https://doi.org/10.1385/1592596878
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 1999
Hardcover ISBN: 978-0-89603-603-1Published: 05 April 1999
Softcover ISBN: 978-1-61737-093-9Published: 09 November 2010
eBook ISBN: 978-1-59259-687-4Published: 01 February 2008
Series ISSN: 1543-1894
Series E-ISSN: 1940-6037
Edition Number: 1
Number of Pages: XI, 238
Topics: Oncology