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Adrenergic Receptor Protocols

  • Book
  • © 2000

Overview

Editors:
  1. Curtis A. Machida

    1. Oregon Regional Primate Research Center, Oregon Health Sciences University, Beaverton

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Part of the book series: Methods in Molecular Biology (MIMB, volume 126)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Genetic Analysis

    1. Front Matter

      Pages 1-1
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    2. Construction of Libraries for Isolation of Adrenergic Receptor Genes

      • Margaret A. Scofield, Jean D. Deupree, David B. Bylund
      Pages 3-35

    3. Isolation of Adrenergic Receptor Genes

      • Margaret A. Scofield, Jean D. Deupree, David B. Bylund
      Pages 37-51

    4. Analyses of Adrenergic Receptor Sequences

      • Jean D. Deupree, Margaret A. Scofield, David B. Bylund
      Pages 53-72

    5. Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes

      • Dianne M. Perez, Michael J. Zuscik
      Pages 73-89

    6. Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones

      • Dianne M. Perez, Michael J. Zuscik
      Pages 91-116

    7. Genetic Polymorphisms of Adrenergic Receptors

      • Ian P. Hall, Amanda P. Wheatley, Jane C. Dewar
      Pages 117-126

  4. Expression Analysis

    1. Front Matter

      Pages 187-187
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    2. Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors

      • Stéphane Schaak, Jean-Christophe Devedjian, Hervé Paris
      Pages 189-206

    3. Expression of β-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells

      • A. Donny Strosberg, Jean-Luc Guillaume
      Pages 207-214

    4. Expression of β-Adrenergic Receptors in E. coli

      • Ralf Jockers, Margaret Scofield
      Pages 215-220

    5. Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors

      • Hongying Zhong, Kenneth P. Minneman
      Pages 221-234

    6. Transient Transfection and Adrenergic Receptor Promoter Analysis

      • Yi-Tang Tseng, James F. Padbury
      Pages 235-239
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About this book

Adrenergic receptors are important modulators in the sympathetic c- trol of various metabolic processes in the central and peripheral nervous s- tems. These receptors are localized at multiple sites throughout the central nervous system (CNS) and serve as important regulators of CNS-mediated behavior and neural functions, including mood, memory, neuroendocrine c- trol, and stimulation of autonomic function. Adrenergic Receptor Protocols consists of 35 chapters dealing with va- ous aspects of adrenergic receptor analyses, including the use of genetic, RNA, protein expression, transactivator, second messenger, immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. This volume details the use of various methods to examine the adrenergic receptor system, using aspects of the genetic flow of information as a guide (DNA? RNA ? transactivator ? protein expression ? second messenger analyses ? cellular analyses ? transgenic whole animal approaches). Adrenergic Receptor Protocols displays step-by-step methods for s- cessful replication of experimental procedures, and would be useful for both experienced investigators and newcomers in the field, including those beg- ning graduate study or undergoing postdoctoral training. The Notes section contained in each chapter provides valuable troubleshooting guides to help develop working protocols for your laboratory. With Adrenergic Receptor Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for analyzing adrenergic receptors. I would like to thank the many chapter authors for their contributions.

Editors and Affiliations

  • Oregon Regional Primate Research Center, Oregon Health Sciences University, Beaverton

    Curtis A. Machida

Bibliographic Information

  • Book Title: Adrenergic Receptor Protocols

  • Editors: Curtis A. Machida

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596843

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2000

  • Hardcover ISBN: 978-0-89603-602-4Published: 05 November 1999

  • Softcover ISBN: 978-1-4899-4156-5Published: 16 August 2013

  • eBook ISBN: 978-1-59259-684-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 577

  • Topics: Neurosciences

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