Molecular Diagnosis of Infectious Diseases

1. Front Matter

   Pages i-xv

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2. Protein-Based Identification Of Antigens

   1. Front Matter

      Pages 1-1

      PDF

   2. Proteomic Approaches to Antigen Discovery

      • Karen M. Dobos, John S. Spencer, Ian M. Orme, John T. Belisle

      Pages 3-17

   3. Immunoproteomics

      • Alexander Krah, Peter R. Jungblut

      Pages 19-32

   4. Immunoprecipitation and Blotting

      • Stephen Thompson

      Pages 33-45

3. DNA-Based Identification of Antigens

   1. Front Matter

      Pages 47-47

      PDF

   2. Representational Difference Analysis of cDNA

      • Lucas D. Bowler

      Pages 49-66

   3. Microarray Data Analysis and Mining

      • Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, Raffaele A. Calogero

      Pages 67-90

   4. Expression Cloning

      • Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, Yasir A. W. Skeiky

      Pages 91-106

4. Identification of Epitopes and Immunomodulatory Components

   1. Front Matter

      Pages 107-107

      PDF

   2. Determination of Epitopes by Mass Spectrometry

      • Christine Hager-Braun, Kenneth B. Tomer

      Pages 109-120

   3. Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays

      • Timothy W. Tobery, Michael J. Caulfield

      Pages 121-132

   4. Virus-like Particles

      • Ludwig Deml, Jens Wild, Ralf Wagner

      Pages 133-157

   5. Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components

      • Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, Wulf Schneider-Brachert

      Pages 159-175

5. Expression of Recombinant Proteins

   1. Front Matter

      Pages 177-177

      PDF

   2. High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format

      • Jutta Drees, Jason Smith, Frank Schäfer, Kerstin Steinert

      Pages 179-190

   3. Production of Antigens in Chlamydomonas reinhardtii

      • Markus Fuhrmann

      Pages 191-195

   4. Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA

      • Marcus Graf, Ludwig Deml, Ralf Wagner

      Pages 197-210

6. Purification, Modification, and Renaturation of Recombinant Proteins

   1. Front Matter

      Pages 211-211

      PDF

   2. Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)

      • Udo Reischl

      Pages 213-224

Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity.

• infectious diseases

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal

"...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book."
-Archives of Pathology and Laboratory Medicine

"...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology

"...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology

"...a very good compilation of techniques that can be used for numerous applications."
-Analytical Biochemistry

"...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes."
-International Journal of Medical Microbiology

"...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal

"...well-organized...provides detailed methods andpractical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases

"...brings together the key elements of oral medicine and nutrition in an accessible form for the dental professional...an excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical Nutrition

Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine

• Accenture GmbH, Munich, Germany

  Jochen Decler

• Institute for Medical Microbiology and Hygiene, University of Regensburg, Regenburg, Germany

  Udo Reischl

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