Access this book
Other ways to access
Table of contents (21 protocols)
-
Chromosomal Techniques
-
Tissue Techniques
Keywords
About this book
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. However, in situ hybridization has retained a reputation as one of the more difficult molecular biological techniques. This may be caused, in part, by the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in this book, by researchers active in the field, should make it possible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique successfully in their laboratories. In Situ Hybridization Protocols aims to provide useful protocols for two groups of researchers who use the technique: those who use in situ hybridi- tion to localize genes to particular chromosomes and those researchers who use DNA or RNA probes to localize expression of mRNA in tissue. This new edition also encompasses a number of techniques not covered in the previous edition, such as in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscopic level, and in situ detection of DNA fragmentation in apoptosis. The final two chapters of the book provide examples of application of in situ hybridization techniques to research and clinical problems.
Reviews
From Reviews of the First Edition by Andy Choo. . .
". . .a very helpful manual. . .essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire."
--Molecular Biotechnology
" . . .a good book for anyone wishing to exploit all that in situ hybridization has to offer."
-- Biopharm Manufacturing
Reviews for Second Edition...
"This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique...The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both."-Heredity
"This book is well written and easy to follow and almost all different applications of the in situ hybridization techniques are covered in this volume...highly recommended..." - Cell Biology International
". . .a very helpful manual. . .essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire."
--Molecular Biotechnology
" . . .a good book for anyone wishing to exploit all that in situ hybridization has to offer."
-- Biopharm Manufacturing
Reviews for Second Edition...
"This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique...The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both."-Heredity
"This book is well written and easy to follow and almost all different applications of the in situ hybridization techniques are covered in this volume...highly recommended..." - Cell Biology International
Editors and Affiliations
Bibliographic Information
Book Title: In Situ Hybridization Protocols
Editors: Ian A. Darby
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592596770
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2000
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIX, 480
Topics: Cell Biology