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Cytokine Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Marc Ley

    1. Laboratory of Biochemistry, Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium

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Part of the book series: Methods in Molecular Biology (MIMB, volume 249)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Large-Scale Generation of Plasmids that Express Type I Interferon

    • Peter Härle, Daniel J. J. Carr
    Pages 1-6

  3. Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay

    • Rebecca J. Chan, Min You, Gen-Sheng Feng
    Pages 7-20

  4. Modulation of the Interferon-γ Signal by Transfection of Cells with an Antisense-RNA Expressing Vector

    • Leen Delrue, Marc De Ley
    Pages 21-29

  5. Competitive RT-PCR to Quantify Small Amounts of mRNA

    • Gabriela Aust
    Pages 31-40

  6. In SituHybridization for Cytokines in Human Tissue Biopsies

    • Emily Mathey, John Pollard, Patricia Armati
    Pages 41-46

  7. Purification, Identification, and Synthesis of Chemokines

    • Patricia Menten, Anja Wuyts, Jo Van Damme
    Pages 47-63

  8. Receptor Isolation and Characterization

    • Daniela Novick, Menachem Rubinstein
    Pages 65-80

  9. Crystallization of Cytokine-Receptor Complexes

    • William J. Cook, Mark R. Walter
    Pages 81-92

  10. Biosensor Analysis of Receptor-Ligand Interactions

    • Sheng-Jiun Wu, Irwin Chaiken
    Pages 93-110

  11. Analysis of SH2 Ligands and Identification of Sites of Interaction

    • Manuel Baca
    Pages 111-120

  12. Assays for Antiviral Activity

    • Anthony Meager
    Pages 121-134

  13. Assays for Cytotoxicity

    • Anthony Meager
    Pages 135-151

  14. Assays for Chemotaxis

    • Anja Wuyts, Patricia Menten, Nancy Van Osselaer, Jo Van Damme
    Pages 153-166

  15. In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor

    • James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, Ikuo Horii
    Pages 167-175

  16. Development of a Mammalian Tet-On Expression Cell Line

    • Yong-Yu Liu, Myles C. Cabot
    Pages 177-192

  17. Immunohistochemical Detection of Cytokines in Human Tissue Sections

    • Sandra Hulshof, Danny F. Dukers, Wim Vos, Corline J. A. De Groot
    Pages 193-199

  18. Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level

    • Karin Loré, Jan Andersson
    Pages 201-218

  19. Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation

    • Howard R. Petty
    Pages 219-227

  20. Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production

    • Etsushi Kuroda, Uki Yamashita
    Pages 229-238
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About this book

Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results.
Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.

Editors and Affiliations

  • Laboratory of Biochemistry, Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium

    Marc Ley

Bibliographic Information

  • Book Title: Cytokine Protocols

  • Editors: Marc Ley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596673

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-0-89603-948-3Published: 08 October 2003

  • Softcover ISBN: 978-1-61737-269-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-667-6Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 240

  • Topics: Immunology

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