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Gene Delivery to Mammalian Cells

Volume 2: Viral Gene Transfer Techniques

  • Book
  • © 2004

Overview

Editors:
  1. William C. Heiser

    1. Bio-Rad Laboratories, Hercules

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Part of the book series: Methods in Molecular Biology (MIMB, volume 246)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Delivery Using Adenoviruses

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Adenovirus-Mediated Gene Delivery

      • Joanne T. Douglas
      Pages 3-14

    3. DNA Delivery to Cells in Culture

      • Miroslava Ogorelkova, Seyyed Mehdy Elahi, David Gagnon, Bernard Massie
      Pages 15-27

    4. Adenovirus-Mediated Gene Delivery to Skeletal Muscle

      • Joanne T. Douglas
      Pages 29-35

    5. Delivery of Adenoviral DNA to Mouse Liver

      • Sheila Connelly, Christine Mech
      Pages 37-52

    6. Delivery of DNA to Lung Airway Epithelium

      • Daniel J. Weiss
      Pages 53-68

    7. Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors

      • Paul N. Reynolds
      Pages 69-89

    8. Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors

      • Joseph M. Alisky, Beverly L. Davidson
      Pages 91-120

    9. Adenovirus-Mediated Gene Transfer to Tumor Cells

      • Manel Cascalló, Ramon Alemany
      Pages 121-138

    10. Adenovirus-Mediated Gene Delivery to Dendritic Cells

      • Laura Timares, Joanne T. Douglas, Bryan W. Tillman, Victor Krasnykh, David T. Curiel
      Pages 139-154

  3. Delivery Using Adeno-Associated Viruses

    1. Front Matter

      Pages 155-155
      Download chapter PDF

    2. Overview of Adeno-Associated Viral Vectors

      • Thomas M. Daly
      Pages 157-165

    3. AAV Vector Delivery to Cells in Culture

      • Andrew Smith, Roy Collaco, James P. Trempe
      Pages 167-177

    4. AAV-Mediated Gene Transfer to Skeletal Muscle

      • Roland W. Herzog
      Pages 179-194

    5. AAV-Mediated Gene Transfer to the Liver

      • Thomas M. Daly
      Pages 195-199

    6. AAV-Mediated Gene Transfer to Mouse Lungs

      • Christine L. Halbert, A. Dusty Miller
      Pages 201-212

    7. Gene Delivery to the Mammalian Heart Using AAV Vectors

      • Danny Chu, Patricia A. Thistlethwaite, Christopher C. Sullivan, Mirta S. Grifman, Matthew D. Weitzman
      Pages 213-224

    8. Gene Delivery to the Mouse Brain with Adeno-Associated Virus

      • Marco A. Passini, Deborah J. Watson, John H. Wolfe
      Pages 225-236

    9. Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus

      • Selvarangan Ponnazhagan, Frank Hoover
      Pages 237-243
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About this book

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

Reviews

For Volumes 1 & 2

"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology

"...clearly and succinctly presented...a must for any biology or medical library." - ASM News

Editors and Affiliations

  • Bio-Rad Laboratories, Hercules

    William C. Heiser

Bibliographic Information

  • Book Title: Gene Delivery to Mammalian Cells

  • Book Subtitle: Volume 2: Viral Gene Transfer Techniques

  • Editors: William C. Heiser

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596509

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-095-3Published: 28 October 2003

  • Softcover ISBN: 978-1-61737-353-4Published: 10 November 2010

  • eBook ISBN: 978-1-59259-650-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 566

  • Topics: Cell Biology

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