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Gene Delivery to Mammalian Cells

Volume 1: Nonviral Gene Transfer Techniques

  • Book
  • © 2004

Overview

Editors:
  1. William C. Heiser

    1. Bio-Rad Laboratories, Hercules

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Part of the book series: Methods in Molecular Biology (MIMB, volume 245)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Delivery Using Chemical Methods

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Chemical Methods for DNA Delivery

      • Dexi Liu, Evelyn F. Chia, Hui Tian
      Pages 3-23

    3. Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran

      • Gregory S. Pari, Yiyang Xu
      Pages 25-32

    4. DNA Delivery to Cells in Culture Using Peptides

      • Lei Zhang, Nicholas Ambulos, A.James Mixson
      Pages 33-52

    5. DNA Delivery to Cells in Culture Using PNA Clamps

      • Todd D. Giorgio, Shelby K. Wyatt
      Pages 53-65

    6. Dendrimer-Mediated Cell Transfection in Vitro

      • James R. Baker Jr., Anna U. Bielinska, Jolanta F. Kukowska-Latallo
      Pages 67-81

    7. DNA Delivery to Cells in Culture Using Cationic Liposomes

      • Shelby K. Wyatt, Todd D. Giorgio
      Pages 83-93

    8. Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium

      • John Marshall, Nelson S. Yew, Seng H. Cheng
      Pages 95-114

    9. Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium

      • Young K. Song, Guisheng Zhang, Dexi Liu
      Pages 115-124

    10. Delivery of DNA to Tumor Cells Using Cationic Liposomes

      • Duen-Hwa Yan, Bill Spohn, Mien-Chie Hung
      Pages 125-135

    11. Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes

      • Lalitha R. Belur, R.Scott McIvor
      Pages 137-143

  3. Delivery Using Physical Methods

    1. Front Matter

      Pages 145-145
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    2. Gene Delivery Using Physical Methods

      • Te-hui W. Chou, Subhabrata Biswas, Shan Lu
      Pages 147-165

    3. Gene Delivery to Mammalian Cells by Microinjection

      • Robert King
      Pages 167-173

    4. Delivery of DNA to Cells in Culture Using Particle Bombardment

      • William C. Heiser
      Pages 175-184

    5. Delivery of DNA to Skin by Particle Bombardment

      • Shixia Wang, Swati Joshi, Shan Lu
      Pages 185-196

    6. Biolistic Transfection of Cultured Organotypic Brain Slices

      • A.Kimberley McAllister
      Pages 197-205

    7. Efficient Electroporation of Mammalian Cells in Culture

      • Peter A. Barry
      Pages 207-213

    8. Delivery of DNA to Skin by Electroporation

      • Nathalie Dujardin, Véronique Préat
      Pages 215-226
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Keywords

About this book

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo.
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

Reviews

For Volumes 1 & 2

"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology

Volume 1

"...provides insights into the choices available in this important area of biology and medicine." - Microbiology Today

Editors and Affiliations

  • Bio-Rad Laboratories, Hercules

    William C. Heiser

Bibliographic Information

  • Book Title: Gene Delivery to Mammalian Cells

  • Book Subtitle: Volume 1: Nonviral Gene Transfer Techniques

  • Editors: William C. Heiser

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596495

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-086-1Published: 28 October 2003

  • Softcover ISBN: 978-1-61737-349-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-649-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 301

  • Topics: Cell Biology

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