Overview
- Editors:
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Howard B. Lieberman
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Center for Radiological Research, Columbia University, New York
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Table of contents (26 protocols)
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Front Matter
Pages i-xiii
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Induction and Detection of Changes in cell Cycle Progression
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- Howard B. Lieberman, Kevin M. Hopkins
Pages 3-10
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- Haiying Hang, Michael H. Fox
Pages 23-35
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- Wei-Hsin Sun, Melvin L. DePamphilis
Pages 37-53
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- Audra Day, Colette Schneider, Brandt L. Schneider
Pages 55-76
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- Hong Zhang, Wolfram Siede
Pages 77-91
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- Eliana B. Gómez, Susan L. Forsburg
Pages 93-111
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Analysis of Genes Involved in Checkpoint Control
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Front Matter
Pages 113-113
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- Sally A. Amundson, Albert J. Fornace Jr.
Pages 125-141
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- Jian Zhang, Lisa Ottmers, Brandt L. Schneider
Pages 143-161
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- Migadlisel Colòn, Nancy C. Walworth
Pages 175-187
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- Jian Qin, Zhe Peng, Maureen V. McLeod
Pages 189-194
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- Erik K. Flemington, Antonio Rodriguez
Pages 195-206
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- Chenguang Wang, Maofu Fu, Richard G. Pestell
Pages 207-216
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Analysis of Proteins Involved in Checkpoint Control
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Front Matter
Pages 217-217
About this book
The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.
Reviews
"...includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls." - Tumori
Editors and Affiliations
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Center for Radiological Research, Columbia University, New York
Howard B. Lieberman
