Practical Cell Culture Techniques

1. Front Matter

   Pages i-xix

   PDF

2. Basic Techniques for Cell Culturing

   • Diane E. Harold, Wolfgang Walz

   Pages 1-20

3. Identification of Cell Types in Neural Cultures

   • Colin J. Barnstable

   Pages 21-62

4. Environmental Influences on Cells in Culture

   • Jane E. Bottenstein

   Pages 63-85

5. Detection and Analysis of Growth Factors Affecting Neural Cells

   • Marston Manthorpe, David Muir, Brigitte Pettmann, Silvio Varon

   Pages 87-137

6. The Role of Cell Adhesion Molecules in Neurite Growth

   • Daren Ure, Ann Acheson

   Pages 139-171

7. Three-Dimensional Organ Culture Systems

   • B. Rogister, J. M. Rigo, P.P. Lefebvre, P. Leprince, P. Delree, D. Martin et al.

   Pages 173-200

8. Organotypic Slice Explant Roller-Tube Cultures

   • Susan Wray

   Pages 201-239

9. Neurons

   • Jeffrey R. Buchhalter, Marc A. Dichter

   Pages 241-268

10. Astrocytes

    • Bernhard H. J. Juurlink, Leif Hertz

    Pages 269-321

11. Oligodendrocytes

    • Jean de Vellis, Araceli Espinosa de los Monteros

    Pages 323-352

12. Brain Capillaries

    • Nika V. Ketis

    Pages 353-378

13. Back Matter

    Pages 379-392

    PDF

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

...a great deal of useful and well-referenced basic information, as well as...methods for a large number of different techniques ...with extensive protocols for specific techniques.-Trends in Neurosciences

• University of Saskatchewan, Saskatoon, Canada

  Alan A. Boulton, Wolfgang Walz

• University of Alberta, Edmonton, Canada

  Glen B. Baker

Policies and ethics