Molecular Neurobiological Techniques

1. Front Matter

   Pages i-xviii

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2. Analysis of Brain mRNAs by Translation in Vitro

   • David R. Colman

   Pages 1-12

3. Preparation of cDNA Libraries and Isolation and Analysis of Specific Clones

   • Sammye Newman, Anthony T. Campagnoni

   Pages 13-48

4. Preparation and Use of Subtractive cDNA Hybridization Probes for cDNA Cloning

   • Gabriel H. Travis, Robert J. Milner, J. Gregor Sutcliffe

   Pages 49-78

5. Analysis of Brain-Specific Gene Products

   • Robert J. Milner, J. Gregor Sutcliffe

   Pages 79-115

6. Isolation and Structure Determination of Genes

   • Wendy B. Macklin, Gelia W. Campagnoni

   Pages 117-151

7. Gene-Mapping Techniques

   • Robert S. Sparkes

   Pages 153-162

8. Methods for Genetic Linkage Analyses

   • M. Anne Spence

   Pages 163-175

9. Lineage Analysis and Immortalization of Neural Cells via Retrovirus Vectors

   • Constance Cepko

   Pages 177-219

10. Transgenic Mice in Neurobiological Research

    • Brian Popko, Carol Readhead, Jessica Dausman, Leroy Hood

    Pages 221-237

11. In Situ Hybridization

    • Michael C. Wilson, Gerald A. Higgins

    Pages 239-284

12. Back Matter

    Pages 285-295

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It goes without saying that the principles and techniques of molecular biology are having and will continue to have a major impact on investigations into nervous system structure and func tion. It is becoming increasingly apparent to neuroscientists in all subdisciplines that a working knowledge of the language, approaches, and techniques of molecular biology is indispensable for their work. For these reasons, the editors have decided to devote this volume of Neuromethods to the techniques of molecular biology and their application to neural systems. There currently exist a number of excellent reference technical manuals that de scribe molecular neurobiological techniques in great detail, and many of these are cited within the chapters included in this volume. It was not the intention of the editors or authors of this volume to duplicate these efforts. Rather, our intention was to present to the neuroscientist who is relatively unfamiliar with these methodologies an understanding of how specific techniques are used to approach major molecular neurobiological problems as well as a set of techniques that work in the laboratories of the individuals writing the chapters. In some cases, there are duplica tions of techniques these have been retained to illustrate the range of variability of the technique and/or the flexibility of the method to study different types of problems. We hope that the chapters will provide the reader with an understanding of the methods and their applicability to neurobiological problems; and, perhaps, suggest new directions for the reader's research efforts. Anthony T.

An excellent set of chapters [on] the molecular biologist's contribution to the neurobiologist's arsenal.-Trends in the Neurosciences

• University of Saskatchewan, Saskatoon, Canada

  Alan A. Boulton

• University of Alberta, Edmonton, Canada

  Glen B. Baker

• University of California, Los Angeles

  Anthony T. Campagnoni

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