The Neuronal Microenvironment

1. Front Matter

   Pages i-xxvi

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2. Cerebrovascular Water and Ion Transport

   • Joseph E. Melton

   Pages 1-31

3. The Choroid Plexus—Arachnoid Membrane—Cerebrospinal Fluid System

   • Conrad E. Johanson

   Pages 33-104

4. Fluid Compartments

   • Asao Hirano, Takeo Kato

   Pages 105-126

5. Physical and Biochemical Methods for Analysis of Fluid Compartments

   • K. G. Go

   Pages 127-185

6. Measurement of Metabolic Activity Associated with Ion Shifts

   • Myron Rosenthal, Thomas J. Sick

   Pages 187-245

7. Use of Ion-Selective Microelectrodes and Voltammetric Microsensors to Study Brain Cell Microenvironment

   • Charles Nicholson, Margaret E. Rice

   Pages 247-361

8. Patch Clamp Recording Methodology

   • Jerry M. Farley

   Pages 363-419

9. Analysis of Ion Fluxes and Fluid Compartmentation in Brain Slices

   • Wolfgang Walz

   Pages 421-440

10. Ion Transport and Volume Measurements in Cell Cultures

    • H. K. Kimelberg, Wolfgang Walz

    Pages 441-492

11. Electrophysiological Methods Applied in Nervous System Cultures

    • H. Kettenmann

    Pages 493-544

12. Electrophysiological Methods for Studying Ionic Currents in Brain Slices and Cell Cultures

    • Brian A. MacVicar, Maeve O’Beirne

    Pages 545-587

13. Calcium Ions

    • R. Pumain

    Pages 589-650

14. Acid—Base Balance

    • Bo K. Siesjö, Fredrik Boris-Möller, Eduardo Martins

    Pages 651-690

15. Magnesium Ions

    • Jerry G. Chutkow

    Pages 691-715

16. Back Matter

    Pages 717-732

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Techniques in the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neuroscientists, and those that are available often seem either out of date or limited in scope. This series is about the methods most widely used by modern-day neuroscientists and is written by their colleagues who are practicing experts. Volume 1 will be useful to all neuroscientists since it concerns those procedures used routmely across the widest range of s- disciplines. Collecting these general techniques together in a single volume strikes us not only as a service, but will no doubt prove of exceptional utilitarian value as well. Volumes 2 and 3 describe all current procedures for the analyses of amines and theirmetabolites and of amino acids, respectively. These collections will clearly be of value to all neurosclentists working m or contemplating research in these fields. Similar reasons exist for Volume 4 on receptor binding techniques, since experimental details are provided for all types of ligand-receptor binding, including chapters on general principles, drug discovery and development, and a most useful appendix on computer programs for Scatchard, nonlinear, and competitive d- placement analyses. Volume 5 provides procedures for the asse- ment of enzymes involved in biogenic amine synthesis and catabolism. Volumes in the NEUROMETHODS series will be useful to neurochemists, -pharmacologists, -physiologists, -anatomlsts, psychopharmacologists, psychiatrists, neurologists, and chemists (organic, analytical, pharmaceutical, medicinal); in fact, everyone involved in the neurosciences, both basic and clinical.

• University of Saskatchewan, Saskatchewan

  Alan A. Boulton

• University of Alberta, Alberta

  Glen B. Baker

• Universdy of Saskatchewan, Saskatchewan

  Wolfgang Walz

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