Molecular Bacteriology: Protocols and Clinical Applications

1. Front Matter

   Pages i-xii

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2. Impact of Molecular Methods on Clinical Bacteriology

   • Robert C. George

   Pages 1-15

3. Genomic DNA Digestion and Ribotyping

   • J. Zoe Jordens

   Pages 17-31

4. Pulsed-Field Gel Electrophoresis

   • Mary Elizabeth Kaufmann

   Pages 33-50

5. Plasmid Analysis

   • Alan P. Johnson, Neil Woodford

   Pages 51-62

6. DNA Amplification

   • Nick A. Saunders, Jonathan P. Clewley

   Pages 63-82

7. Arbitrarily Primed PCR Methods for Studying Bacterial Diseases

   • David Ralph, Michael McClelland

   Pages 83-102

8. Genomic Fingerprinting by Application of rep-PCR

   • Anne M. Ridley

   Pages 103-115

9. Molecular Approaches to the Identification of Streptococci

   • Robert E. McLaughlin, Joseph J. Ferretti

   Pages 117-138

10. Pneumococcal Diseases

    • Anthony M. Smith, Keith P. Klugman

    Pages 139-156

11. Molecular Approaches in Mycobacterium tuberculosisand Other Infections Caused by Mycobacterium Species

    • Madhu Goyal, Douglas Young

    Pages 157-190

12. Diagnosis and Epidemiology of Diphtheria

    • Androulla Efstratiou, Kathryn H. Engler, Aruni de Zoysa

    Pages 191-212

13. Diagnosis and Epidemiology of Infections Caused by Legionella spp.

    • Norman K. Fry, Timothy G. Harrison

    Pages 213-242

14. Molecular Methods for Haemophilus influenzae

    • Mark A. Herbert, Derrick Crook, E. Richard Moxon

    Pages 243-263

15. The Impact o Molecular Techniques on the Study of Meningococcal Disease

    • Martin C. J. Maiden

    Pages 265-291

16. Gonorrhea

    • Catherine A. Lson

    Pages 293-308

17. Chancroid

    • Stephen A. Morse, David L. Trees, Patricia A. Totten

    Pages 309-333

18. Mycoplasma and Ureaplasma Infections

    • Claire B. Gilroy, David Taylor-Robinson

    Pages 335-354

19. Application of Molecular Methods to the Study of Infections Caused by Salmonella spp.

    • E. John Threlfall, Mike D. Hampton, Anne M. Ridley

    Pages 355-368

20. Cholera

    • Timothy J. Barrett, Daniel N. Cameron

    Pages 369-385

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

• DNA
• Escherichia coli
• PCR
• antibiotics
• bacteria
• bacterial infection
• bacterial infections
• bacteriology
• infection
• infections
• infectious
• infectious disease
• infectious diseases
• nosocomial infection

"The book is authoritative, as its many contributors are all experts in their fields; their contributions have been well coordinated by the editors, resulting in a unified work. . .The book is sturdily bound in hardback to withstand the considerable use it deserves and will undoubtedly get. It represents very good value and I recommend that all laboratories interested in molecular diagnosis in bacteriology should buy a copy of it."-Journal of Medical Microbiology

"Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories...Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations....This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases."-Doody's Health Science Book Review Journal

". . .a book that will be useful not only to the experienced researchers, but also to the newcomer."-Laboratoriums Medizin

• Central Public Health Laboratory, London, UK

  Neil Woodford, Alan P. Johnson

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