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Molecular Diagnosis of Infectious Diseases

  • Book
  • © 1998

Overview

Editors:
  1. Udo Reischl

    1. University of Regensburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 13)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Recombinant Proteins and Peptide

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Identification of Important Antigens

      1. Identification of Novel Diagnostic Markers by Differential Display
        • Peng Liang, Feilan Wang, Weimin Zhu, Robert P. O’Connell, Lidia Averboukh
        Pages 3-13
      2. Immunoprecipitation
        • Kari Johansen, Lennart Svensson
        Pages 15-28

    3. Identification of Corresponding Open Reading Frames

      1. RT-PCR Methods and Applications
        • Paul D. Siebert
        Pages 29-53
      2. Quantitative RT-PCR
        • Paul D. Siebert
        Pages 55-79
      3. Cloning cDNA Ends Using RACE
        • Yue Zhang, Michael A. Frohman
        Pages 81-105

    4. Identification of Important Antigenic Epitopes

      1. A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains
        • Robert C. Mierendorf, Beth Hammer, Robert E. Novy
        Pages 107-123
      2. Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis
        • Ulla Wienhues, Wolfgang Kraas, Christoph Seidel, Hans Georg Ihlenfeidt, Günther Jung
        Pages 125-142

    5. Cloning and Expression of Open Reading Frames in Prokaryontic Systems

      1. Amplification of Genomic DNA by PCR
        • Bruno Frey
        Pages 143-156
      2. PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in <i>Escherichia coli</i>
        • Udo Reischl
        Pages 157-168
      3. Application of TEV Protease in Protein Production
        • Deborah A. Polayes, T. Dawn Parks, Stephen A. Johnston, William G. Dougherty
        Pages 169-183

    6. Cloning and Expression of Open Reading Frames in Eukaryontic Systems

      1. Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic, Therapeutic, and Preventive Purposes
        • Ludwig Deml, Ralf Wagner
        Pages 185-200
      2. Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked Immunosorbent Assays
        • Alan Warnes, Anthony R. Fooks, John R. Stephenson
        Pages 201-211
      3. Generation of Recombinant Baculovirus DNA in <i>E.coli</i> Using a Baculovirus Shuttle Vector
        • Valentina C. Ciccarone, Deborah A. Polayes, Verne A. Luckow
        Pages 213-235
      4. The SFV Gene Expression System
        • Valentina C. Ciccarone, Joel A. Jessee, Peter Liljeström
        Pages 237-255

    7. Purification of Recombinant Proteins

      1. Expression and Purification of Recombinant Proteins Using the pET System
        • Robert C. Mierendorf, Barbara B. Morris, Beth Hammer, Robert E. Novy
        Pages 257-292
      2. Procedures for the Analysis and Purification of His-Tagged Proteins
        • Richard E. Kneusel, Joanne Crowe, Melanie Wulbeck, Joachim Ribbe
        Pages 293-308
      3. Detection and Immobilization of Proteins Containing the 6xHis Tag
        • Richard E. Kneusel, Melanie Wulbeck, Joachim Ribbe
        Pages 309-322
      4. The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
        • Louis E. C. Leong
        Pages 323-329
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Keywords

About this book

In most societies the medical field is undergoing a dramatic reorien- tion. Fundamentally new technologies in diagnosis and therapy, as well as the extension of life expectations, have increased health costs to an extent that now nears the limits of acceptability. One consequence has been a reevaluation of the need for and duration of each individual hospitalization. For diagnostic laboratories, results must now be obtained rapidly in order to include them in therapeutic decisions. Furth- more, therapeutic approaches to the control of infectious agents, limited by the number of therapeutic compounds available and by the growing perce- age of multiresistant agents, now require improvement in the precision of diagnostic approaches. The necessity for maximal reduction of the risk of infection by the development of appropriate pharmaceutical products demands even higher levels of sensitivity in diagnostic tests. Today's highly purified antigens---often from recombinantly generated antigens or peptides, monoclonal or recombinant antibodies, and more sen- tive signal-production and signal-detection systems--have greatly advanced immunologically based detection methodology. However, this approach has biological limits that further enhancement of sensitivity cannot breach. Fortunately nucleic acids have now been quite successfully added to the sp- trum of diagnostic targets. Recombinant-produced antigens and the integration of microelectronics and microfluidics offer new perspectives and possibilities.

Reviews

Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source."-Archives of Pathology and Laboratory Medicine

Editors and Affiliations

  • University of Regensburg, Germany

    Udo Reischl

Bibliographic Information

  • Book Title: Molecular Diagnosis of Infectious Diseases

  • Editors: Udo Reischl

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896034852

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-597-6Published: 18 January 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 629

  • Number of Illustrations: 126 b/w illustrations

  • Topics: Infectious Diseases

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